Screening and identification of nucleocapsid protein-nanobodies that inhibited Newcastle disease virus replication in DF-1 cells

被引:3
|
作者
Fan, Wenqi [1 ,2 ]
Ji, Pinpin [1 ,2 ]
Sun, Xuwen [1 ,2 ]
Kong, Min [1 ,2 ]
Zhou, Ning [1 ,2 ]
Zhang, Qiang [1 ,2 ]
Wang, Ying [1 ,2 ]
Liu, Qianqian [1 ,2 ]
Li, Xiaoxuan [1 ,2 ]
Zhou, En-Min [1 ,2 ]
Zhao, Qin [1 ,2 ]
Sun, Yani [1 ,2 ]
机构
[1] Northwest Agr & Forestry Univ, Coll Vet Med, Dept Prevent Vet Med, Xianyang, Peoples R China
[2] Minist Agr, Sci Observing & Expt Stn Vet Pharmacol & Diagnost, Yangling, Peoples R China
基金
国家重点研发计划;
关键词
nanobody; Newcastle disease virus; nucleocapsid protein; viral replication; inhibition; ANTIBODIES; STRAINS; CHINA;
D O I
10.3389/fmicb.2022.956561
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Newcastle disease (ND) is an acute and highly contagious infectious disease found in poultry. Although commercial ND virus (NDV) vaccines are universally used, some case reports persistently documented vaccination failure. Therefore, novel strategies are still required to control the occurrence of the disease in chickens. Recently, nanobodies (Nbs), which have the advantages of small molecular weight and low production costs, have been shown to be promising therapeutics against viral infection. In the present study, a total of 16 Nbs against NDV nucleocapsid protein (NP) were screened from two libraries against NDV using phage display technology. Of the 16 screened Nbs, eight were prevented from binding to NDV NP protein through administering positive chicken sera for anti-NDV antibodies, indicating that the epitopes recognized by these eight Nbs were able to induce the immune response after the chickens were infected with NDV stock. Subsequently, transfection assay, construction of recombinant DF-1 cells capable of expressing different nanobodies and viral inhibition assay were used to screen the nanobodies inhibiting NDV replication. The results demonstrated that Nb18, Nb30, and Nb88 significantly inhibited the replication of Class I and different genotypes of Class II NDV strains in DF-1 cells when they were expressed in the cytoplasm. Collectively, these nanobodies provided new tools for researching the functions of NDV NP protein and may be used as a novel strategy for designing drugs against NDV infection in chickens.
引用
收藏
页数:12
相关论文
共 48 条
  • [31] Newcastle disease virus V protein interacts with hnRNP H1 to promote viral replication
    Tong, Lina
    Chu, Zhili
    Gao, Xiaolong
    Yang, Mengqing
    Adam, Fathalrhman Eisa A.
    Theodore, Daguia Wenam Prince
    Liu, Haijin
    Wang, Xinglong
    Xiao, Sa
    Yang, Zengqi
    VETERINARY MICROBIOLOGY, 2021, 260
  • [32] STRUCTURAL PROTEINS OF NEWCASTLE-DISEASE VIRUS .1. IDENTIFICATION OF A MINOR INTERNAL PROTEIN
    IINUMA, M
    SIMPSON, RW
    VIROLOGY, 1974, 61 (01) : 229 - 239
  • [33] Differential Expression Profile of Chicken Embryo Fibroblast DF-1 Cells Infected with Cell-Adapted Infectious Bursal Disease Virus
    Hui, Raymond K.
    Leung, Frederick C.
    PLOS ONE, 2015, 10 (06):
  • [34] Newcastle Disease Virus V Protein Promotes Viral Replication in HeLa Cells through the Activation of MEK/ERK Signaling
    Chu, Zhili
    Ma, Jiangang
    Wang, Caiying
    Lu, Kejia
    Li, Xiaoqin
    Liu, Haijin
    Wang, Xinglong
    Xiao, Sa
    Yang, Zengqi
    VIRUSES-BASEL, 2018, 10 (09):
  • [35] Fusion of the immunodominant regions of VP1 of human enterovirus 71 to the C-terminal end of the nucleocapsid protein of newcastle disease virus
    Sivasamugham, LA
    Cardosa, MJ
    Tan, WS
    Yusoff, K
    JOURNAL OF CLINICAL VIROLOGY, 2003, 28 : S105 - S106
  • [36] Comparative study of the replication of infectious bursal disease virus in DF-1 cell line and chicken embryo fibroblasts evaluated by a new real-time RT-PCR
    Wang, Yongqiang
    Qi, Xiaole
    Gao, Honglei
    Gao, Yulong
    Lin, Huan
    Song, Xiuqing
    Pei, Lei
    Wang, Xiaomei
    JOURNAL OF VIROLOGICAL METHODS, 2009, 157 (02) : 205 - 210
  • [37] Epigenetic Upregulation of Chicken MicroRNA-16-5p Expression in DF-1 Cells following Infection with Infectious Bursal Disease Virus (IBDV) Enhances IBDV-Induced Apoptosis and Viral Replication
    Duan, Xueyan
    Zhao, Mingliang
    Wang, Yongqiang
    Li, Xiaoqi
    Cao, Hong
    Zheng, Shijun J.
    JOURNAL OF VIROLOGY, 2020, 94 (02)
  • [38] Marek's disease virus type 1 encoded analog of miR-155 promotes proliferation of chicken embryo fibroblast and DF-1 cells by targeting hnRNPAB
    Dang, Lu
    Teng, Man
    Li, Hui-Zhen
    Ma, Sheng-Ming
    Lu, Qing-Xia
    Hao, Hui-Fang
    Zhao, Dong
    Zhou, En-Min
    Zhang, Gai-Ping
    Luo, Jun
    VETERINARY MICROBIOLOGY, 2017, 207 : 210 - 218
  • [39] Induction of apoptosis in Vero cells by Newcastle disease virus requires viral replication, de-novo protein synthesis and caspase activation
    Ravindra, P. V.
    Tiwari, Ashok K.
    Ratta, Barkha
    Chaturvedi, Uttara
    Palia, Sudesh Kumar
    Subudhi, Prasant Kumar
    Kumar, Rajiv
    Sharma, Bhaskar
    Rai, Anant
    Chauhan, R. S.
    VIRUS RESEARCH, 2008, 133 (02) : 285 - 290
  • [40] Both Foot-and-Mouth Disease Virus and Bovine Viral Diarrhea Virus Replication are Inhibited by Mx1 Protein Originated from Porcine
    Shi, Huijun
    Fu, Qiang
    Ren, Yan
    Wang, Dawei
    Qiao, Jun
    Wang, Pengyan
    Zhang, Hui
    Chen, Chuangfu
    ANIMAL BIOTECHNOLOGY, 2015, 26 (01) : 73 - 79