Separation of lac dye components by high-speed counter-current chromatography

被引：42
|
作者：
Oka, H
Ito, Y
Yamada, S
Kagami, T
Hayakawa, J
Harada, K
Atsumi, E
Suzuki, M
Suzuki, M
Odani, H
Akahori, S
Maeda, K
Nakazawa, H
Ito, Y
机构：
[1] Aichi Prefectural Inst Publ Hlth, Kita Ku, Nagoya, Aichi 462, Japan
[2] Dept Hlth, Food Safety & Vet Affairs Div, Naka Ku, Nagoya, Aichi 46001, Japan
[3] Meijo Univ, Fac Pharm, Tempaku Ku, Nagoya, Aichi 468, Japan
[4] Nagoya Univ, Branch Hosp, Dept Internal Med, Nagoya, Aichi 461, Japan
[5] Hoshi Univ, Fac Pharmaceut Sci, Shinagawa Ku, Tokyo 142, Japan
[6] NHLBI, Biophys Chem Lab, NIH, Bethesda, MD 20892 USA
来源：
JOURNAL OF CHROMATOGRAPHY A | 1998年 / 813卷 / 01期
关键词：
counter-current chromatography; food analysis; laccaic acids; lac dye; dyes;
D O I：
10.1016/S0021-9673(98)00311-2
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
High-speed counter-current chromatography has been successfully applied to the separation of the lac dye components. A 25-mg quantity of the sample was separated using a two-phase solvent system composed of tert.-butyl methyl ether-n-butanol-acetonitrile-water (2.2:1:5). The fractions were analyzed by high-performance liquid chromatography and electrospray tandem mass spectrometry. The separation yielded 2.6 mg of 97.2% purl laccaic acid C, 9.5 mg of 98.1% pure laccaic acid A, 3.6 mg of 98.2% pure laccaic acid B, and 0.5 mg of a 95.0% pure anthraquinonedicarboxylic acid with a molecular mass of 360. (C) 1998 Elsevier Science B.V. All rights reserved.
引用
收藏
页码：71 / 77
页数：7
相关论文
共 50 条
  • [1] Separation of ivermectin components by high-speed counter-current chromatography
    Oka, H
    Ikai, Y
    Hayakawa, J
    Harada, K
    Suzuki, M
    Shimizu, A
    Hayashi, T
    Takeba, K
    Nakazawa, H
    Ito, Y
    [J]. JOURNAL OF CHROMATOGRAPHY A, 1996, 723 (01) : 61 - 68
  • [2] Separation of spiramycin components using high-speed counter-current chromatography
    Oka, H
    Harada, K
    Suzuki, M
    Ito, Y
    [J]. JOURNAL OF CHROMATOGRAPHY A, 2000, 903 (1-2) : 93 - 98
  • [3] Preparative separation of isoflavone components in soybeans using high-speed counter-current chromatography
    Du, QZ
    Li, ZH
    Ito, Y
    [J]. JOURNAL OF CHROMATOGRAPHY A, 2001, 923 (1-2) : 271 - 274
  • [4] Separation of lac dye components using high-speed countercurrent chromatography
    Oka, H
    Ito, Y
    [J]. AMERICAN LABORATORY, 2000, 32 (08) : 24 - +
  • [5] Separation of lac dye components using high-speed countercurrent chromatography
    [J]. 2000, Int Sci Commun Inc, Shelton, CT, USA (32)
  • [6] Application of silver ion in the separation of macrolide antibiotic components by high-speed counter-current chromatography
    Wen, Yaoming
    Wang, Jiaoyan
    Chen, Xiuming
    Le, Zhanxian
    Chen, Yuxiang
    Zheng, Wei
    [J]. JOURNAL OF CHROMATOGRAPHY A, 2009, 1216 (22) : 4668 - 4672
  • [7] Preparative separation of the naphthopyranone glycosides by high-speed counter-current chromatography
    dos Santos, LC
    Vilegas, W
    [J]. JOURNAL OF CHROMATOGRAPHY A, 2001, 915 (1-2) : 259 - 263
  • [8] Preparative separation of pyrrolizidine alkaloids by high-speed counter-current chromatography
    Cooper, RA
    Bowers, RJ
    Beckham, CJ
    Huxtable, RJ
    [J]. JOURNAL OF CHROMATOGRAPHY A, 1996, 732 (01) : 43 - 50
  • [9] SEPARATION OF AVERMECTIN COMPONENTS FROM Streptomyces avemitilis EXTRACTION USING HIGH-SPEED COUNTER-CURRENT CHROMATOGRAPHY
    Su, Weike
    Hou, Zhuoni
    Liang, Xianrui
    [J]. CHEMICAL INDUSTRY & CHEMICAL ENGINEERING QUARTERLY, 2013, 19 (04) : 563 - 571
  • [10] Application of high-speed counter-current chromatography to the separation of coumarin and related compounds
    Shibusawa, Y
    Hagiwara, Y
    Chao, ZM
    Ma, Y
    Ito, Y
    [J]. JOURNAL OF CHROMATOGRAPHY A, 1997, 759 (1-2) : 47 - 53
12345