Separation of spiramycin components using high-speed counter-current chromatography

被引：31
|
作者：
Oka, H [1 ]
Harada, K
Suzuki, M
Ito, Y
机构：
[1] Aichi Prefectural Inst Publ Hlth, Kita Ku, Nagoya, Aichi 4628576, Japan
[2] Meijo Univ, Fac Pharm, Tempaku Ku, Nagoya, Aichi 4688503, Japan
[3] NHLBI, Biophys Chem Lab, NIH, Bethesda, MD 20892 USA
来源：
JOURNAL OF CHROMATOGRAPHY A | 2000年 / 903卷 / 1-2期
关键词：
counter-current chromatography; spiramycins; macrolide antibiotics; antibiotics;
D O I：
10.1016/S0021-9673(00)00903-1
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
High-speed counter-current chromatography was successfully applied to the separation of the main components of spiramycin. A 25-mg quantity of sample was separated using a two-phase solvent system composed of n-hexane-ethyl acetate-methanol-water (3:6:5:5), and the fractions were analyzed by high-performance liquid chromatography and fast atom bombardment mass spectrometry. The separation yielded 13.4, 0.7 and 1.7 mg of spiramycins I, II, and III with purities of 98.2, 92.3 and 97.4%, respectively. (C) 2000 Elsevier Science BN. All rights reserved.
引用
收藏
页码：93 / 98
页数：6
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