MicroRNA-3200-3p targeting CAMK2A modulates the proliferation and metastasis of glioma in vitro

被引:5
|
作者
Wang, Haibin [1 ]
Zeng, Zhaobin [1 ]
Yi, Renhui [1 ]
Luo, Jun [1 ]
Chen, Jinming [1 ]
Lou, Jianyun [1 ]
机构
[1] Gannan Med Coll, Dept Neurosurg, Affiliated Hosp 1, 23 Qingnian Rd, Ganzhou 341000, Jiangxi, Peoples R China
关键词
Glioma; miR-3200-3p; CAMK2A; proliferation; metastasis; MALIGNANT GLIOMAS; CELLS;
D O I
10.1080/21655979.2022.2048995
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
MicroRNA (miRNA) is strongly interrelated with the pathogenesis of glioma. However, its potential biological effect and underlying mechanism of miR-3200-3p in human glioma remain elusive. In the current study, we checked the level of miR-3200-3p in different glioma cells. Then, its biological functions on glioma cell proliferation metastasis was investigated using the miR-3200-3p mimic and inhibitor. The direct target of miR-3200-3p was tested in these cells. Results demonstrated that miR-3200-3p is remarkably downregulated in human glioma cells. The relative level of miR-3200-3p is strongly associated with biological features, including proliferation, colony formation, and metastasis. Additionally, Ca2+/calmodulin dependent kinase 2a (CAMK2A) might be the direct target gene of miR-3200-3p, and CAMK2A overexpression reversed the anticancer roles of miR-3200-3p on glioma cellular function. Importantly, these results further showed that miR-3200-3p downregulated the proliferation and metastasis by suppressing the expression of CAMK2A, thus regulating the Ras/Raf/MEK/ERK pathway. This study provided provided insights into the biological role of miR-3200-3p, which might function as a potential biomarker in glioma therapy. [GRAPHICS]
引用
收藏
页码:7785 / 7797
页数:13
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