Cytosolic NADP-isocitrate dehydrogenase in Arabidopsis leaves and roots

被引：24
|
作者：
Leterrier, M. [1 ]
Barroso, J. B. [2 ]
Palma, J. M. [1 ]
Corpas, F. J. [1 ]
机构：
[1] CSIC, Dept Bioquim Biol Celular & Mol Plantas, Estn Expt Zaidin, E-18080 Granada, Spain
[2] Univ Jaen, Dept Bioquim & Biol Mol, Grp Senalizac Mol & Sistemas Antioxidantes Planta, Unidad Asociada CSIC, E-23071 Jaen, Spain
来源：
BIOLOGIA PLANTARUM | 2012年 / 56卷 / 04期
关键词：
hydrogen peroxide; nitric oxide; peroxynitrite; reactive nitrogen species; S-nitrosoglutathione; NITRIC-OXIDE; REDOX HOMEOSTASIS; PEA-PLANTS; STRESS; PURIFICATION; EXPRESSION; SYNTHASE; ENZYME; INACTIVATION; ASSIMILATION;
D O I：
10.1007/s10535-012-0244-6
中图分类号：
Q94 [植物学];
学科分类号：
071001 ;
摘要：
NADP-dependent isocitrate dehydrogenase (NADP-ICDH) catalyses the production of NADPH, which is an essential component in the cellular homeostasis. In Arabidopsis, the kinetic parameters (K (m) and V (max)) of cytosolic NADP-ICDH were different in leaves and roots. In vitro applied H2O2 did not affect the NADP-ICDH activity in either organ, however, the reduced glutathione inhibited the activity in leaves but not in roots. On the other hand, S-nitrosoglutathione (a NO donor) and peroxynitrite depressed NADP-ICDH activity in leaves and roots.
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页码：705 / 710
页数：6
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