Amperometric method for the determination of myoglobin based on the electrochemical reduction on the glassy carbon electrode

被引：7
|
作者：
Yan, F [1 ]
Ruan, CM [1 ]
Chen, XH [1 ]
Deng, JQ [1 ]
Kong, JL [1 ]
机构：
[1] Fudan Univ, Dept Chem, Shanghai 200437, Peoples R China
来源：
FRESENIUS JOURNAL OF ANALYTICAL CHEMISTRY | 1999年 / 363卷 / 01期
基金：
中国国家自然科学基金;
关键词：
D O I：
10.1007/s002160051142
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
An irreversible reduction peak of oxymyoglobin (MbO(2)) was observed on the bare glassy carbon electrode (GCE) in acetate buffer solution under atmospheric conditions. It is the reduction of bonded oxygen in Mb, but not the heme Fe(III)/Fe(II) redox couple that underwent electrochemical reaction on the electrode. The peak current achieved a maximum value in acetate buffer solution of pH 4.0. The peak potential was pH dependent, suggesting that the proton was involved in the electrochemical reaction. Furthermore, the peak current was linearly related to the concentration of myoglobin in the range of 2.5 x 10(-8) similar to 1.0 x 10(-6) mol.L-1 with a detection limit of 5 x 10(-9) mol.L-1.
引用
收藏
页码：83 / 87
页数：5
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