Real-Time Quantitative PCR Detection of Mycobacterium avium Subspecies in Meat Products

被引：31
|
作者：
Klanicova, B. [1 ]
Slana, I. [1 ]
Vondruskova, H. [1 ]
Kaevska, M. [1 ]
Pavlik, I. [1 ]
机构：
[1] Vet Res Inst, Brno 62100, Czech Republic
来源：
JOURNAL OF FOOD PROTECTION | 2011年 / 74卷 / 04期
关键词：
HEAT INACTIVATION; PARATUBERCULOSIS; CULTURE; MILK; RAW;
D O I：
10.4315/0362-028X.JFP-10-332
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
The aim of this work was to examine various purchased meat products and to find out if any traces of Mycobacterium avium subsp. avium, M. avium subsp. hominissuis, and M. avium subsp. paratuberculosis could be detected in these samples. Analysis of the meat products (raw, cooked, and fermented) was performed using a real-time quantitative PCR (qPCR) method for the detection of specific insertion sequences: duplex qPCR for the detection of IS900 specific for M. avium subsp. paratuberculosis, and triplex qPCR for the detection of IS901 specific for Mycobacterium avium subsp. avium and IS1245 specific for M. avium subsp. hominissuis. Of the 77 analyzed meat samples, 17 (22%) were found to contain M. avium subsp. paratuberculosis DNA, 4 (5%) samples contained Mycobacterium avium subsp. avium DNA, and in 12 (16%) samples M. avium subsp. hominissuis DNA was detected. The concentration of M. avium subsp. paratuberculosis and M. avium subsp. hominissuis DNA in some meat products exceeded 10(4) genomes per g. Culture examination of these mycobacterial subspecies was negative. By analyzing a range of meat products, we have provided evidence to support the hypothesis that M. avium is present in everyday commodities sold to the general public.
引用
下载
收藏
页码：636 / 640
页数：5
相关论文
共 50 条
  • [11] Correlation between culture and quantitative real-time PCR results for Mycobacterium avium subspecies paratuberculosis in pooled fecal and environmental samples
    Aly, S. S.
    Mangold, B. L.
    Whitlock, R. H.
    Sweeney, R. W.
    Anderson, R. J.
    Jiang, J.
    Shukken, Y. H.
    Hovingh, E. P.
    Wolfgang, D. R.
    Van Kessel, J. S.
    Karns, J. S.
    Lombard, J. E.
    Smith, J. M.
    Gardner, I. A.
    JOURNAL OF DAIRY SCIENCE, 2010, 93 : 257 - 257
  • [12] Detection of Mycobacterium immunogenum by real-time quantitative Taqman PCR
    Rhodes, Glenn
    Fluri, Alexandra
    Gerber, Marco
    Henderson, Alan
    Ruefenacht, Andrea
    Pickup, Roger W.
    JOURNAL OF MICROBIOLOGICAL METHODS, 2008, 73 (03) : 266 - 268
  • [13] New PCR systems to confirm real-time PCR detection of Mycobacterium avium subsp paratuberculosis
    Herthnek, David
    Bolske, Goran
    BMC MICROBIOLOGY, 2006, 6 (1)
  • [14] Isolation of Listeria monocytogenes dna from meat products for quantitative detection by real-time PCR
    Rodriguez-Lazaro, David
    Hernandez, Marta
    JOURNAL OF RAPID METHODS AND AUTOMATION IN MICROBIOLOGY, 2006, 14 (04): : 395 - 404
  • [15] Quantitative Detection of raw sheep in Meat Products by a Taq Man Real-Time PCR Assay
    Xu Qiong
    Gu Wenjia
    Zhang Yinan
    Zhang Qingping
    Tao Fei
    Hu Xuelian
    Zhao Yan
    Duan Wenfeng
    Qu Qinfen
    PROCEEDINGS OF THE INTERNATIONAL CONFERENCE ON CHEMICAL, MATERIAL AND FOOD ENGINEERING, 2015, 22 : 39 - 42
  • [16] Detection of Mycobacterium avium Subspecies Paratuberculosis in Pooled Fecal Samples by Fecal Culture and Real-Time PCR in Relation to Bacterial Density
    Wichert, Annika
    Einax, Esra
    Hahn, Natalie
    Klassen, Anne
    Donat, Karsten
    ANIMALS, 2021, 11 (06):
  • [17] New PCR systems to confirm real-time PCR detection of Mycobacterium avium subsp. paratuberculosis
    David Herthnek
    Göran Bölske
    BMC Microbiology, 6
  • [18] Quantitative real-time PCR and culture examination of Mycobacterium avium subsp paratuberculosis at farm level
    Hanifian, Shahram
    Khani, Sajjad
    Barzegari, Abolfazl
    Shayegh, Jalal
    VETERINARY MICROBIOLOGY, 2013, 162 (01) : 160 - 165
  • [19] Development of a real-time PCR protocol for the detection of chicken DNA in meat products
    Abitayeva, Gulyaim
    Abeev, Arman
    PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY, 2024, 54 (08): : 1068 - 1078
  • [20] Detection of adulterated murine components in meat products by TaqMan© real-time PCR
    Fang, Xin
    Zhang, Chi
    FOOD CHEMISTRY, 2016, 192 : 485 - 490
12345