Composition, diversity and function of intestinal microbiota in pacific white shrimp (Litopenaeus vannamei) at different culture stages

被引:111
|
作者
Zeng, Shenzheng [1 ]
Huang, Zhijian [1 ]
Hou, Dongwei [1 ]
Liu, Jian [1 ]
Weng, Shaoping [2 ]
He, Jianguo [1 ,2 ]
机构
[1] Sun Yat Sen Univ, Sch Marine Sci, State Key Lab Biocontrol, Guangdong Prov Key Lab Marine Resources & Coastal, Guangzhou, Guangdong, Peoples R China
[2] Sun Yat Sen Univ, Sch Life Sci, Guangzhou, Guangdong, Peoples R China
来源
PEERJ | 2017年 / 5卷
关键词
Intestinal microbiota; Microbial composition; Pacific white shrimp; Diversity; Function; Culture stage; GUT MICROBIOTA; BACTERIAL DIVERSITY; GENE-SEQUENCES; AQUACULTURE; COMMUNITY; WATER; CARP; PROBIOTICS; VARIANCE; READS;
D O I
10.7717/peerj.3986
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Intestinal microbiota is an integral component of the host and plays important roles in host health. The pacific white shrimp is one of the most profitable aquaculture species commercialized in the world market with the largest production in shrimp consumption. Many studies revealed that the intestinal microbiota shifted significantly during host development in other aquaculture animals. In the present study, 22 shrimp samples were collected every 15 days from larval stage (15 day post-hatching, dph) to adult stage (75 dph) to investigate the intestinal microbiota at different culture stages by targeting the V4 region of 16S rRNA gene, and the microbial function prediction was conducted by PICRUSt. The operational taxonomic unit (OTU) was assigned at 97% sequence identity. A total of 2,496 OTUs were obtained, ranging from 585 to 1,239 in each sample. Forty-three phyla were identified due to the classifiable sequence. The most abundant phyla were Proteobacteria, Cyanobacteria, Tenericutes, Fusobacteria, Firmicutes, Verrucomicrobia, Bacteroidetes, Planctomycetes, Actinobacteria and Chloroflexi. OTUs belonged to 289 genera and the most abundant genera were Candidatus_Xiphinematobacter, Propionigenium, Synechococcus, Shewanella and Cetobacterium. Fifty-nine OTUs were detected in all samples, which were considered as the major microbes in intestine of shrimp. The intestinal microbiota was enriched with functional potentials that were related to transporters, ABC transporters, DNA repair and recombination proteins, two component system, secretion system, bacterial motility proteins, purine metabolism and ribosome. All the results showed that the intestinal microbial composition, diversity and functions varied significantly at different culture stages, which indicated that shrimp intestinal microbiota depended on culture stages. These findings provided new evidence on intestinal microorganism microecology and greatly enhanced our understanding of stage-specific community in the shrimp intestinal ecosystem.
引用
收藏
页数:20
相关论文
共 50 条
  • [1] Metagenomic insights into the structure and function of intestinal microbiota of the farmed Pacific white shrimp (Litopenaeus vannamei)
    Gao, Shuo
    Pan, Luqing
    Huang, Fei
    Song, Mengsi
    Tian, Changcheng
    Zhang, Mengyu
    [J]. AQUACULTURE, 2019, 499 : 109 - 118
  • [2] Changes in the intestinal microbiota of Pacific white shrimp (Litopenaeus vannamei) with different severities of Enterocytozoon hepatopenaei infection
    Shen, Hui
    Dou, Yabin
    Li, Haolan
    Qiao, Yi
    Jiang, Ge
    Wan, Xihe
    Cheng, Jie
    Fan, Xianping
    Li, Hui
    Wang, Libao
    Shi, Wenjun
    Qin, Yali
    Shen, Jing
    [J]. JOURNAL OF INVERTEBRATE PATHOLOGY, 2022, 191
  • [3] Characterization of the intestinal microbiota in Pacific white shrimp, Litopenaeus vannamei, fed diets with different lipid sources
    Zhang, Meiling
    Sun, Yuhong
    Chen, Ke
    Yu, Na
    Zhou, Zhigang
    Chen, Liqiao
    Du, Zhenyu
    Li, Erchao
    [J]. AQUACULTURE, 2014, 434 : 449 - 455
  • [4] Microbiota from Litopenaeus vannamei: digestive tract microbial community of Pacific white shrimp (Litopenaeus vannamei)
    Tuyub Tzuc, Jaqueline
    Rendiz Escalante, Diana
    Rojas Herrera, Rafael
    Gaxiola Cortes, Gabriela
    Arena Ortiz, Maria Leticia
    [J]. SPRINGERPLUS, 2014, 3 : 1 - 10
  • [5] Effects of nanoplastics on the gut microbiota of Pacific white shrimp Litopenaeus vannamei
    Zhu, Chenxi
    Li, Yiming
    Liu, Guoxing
    Abdullah, Anisah Lee
    Jiang, Qichen
    [J]. PEERJ, 2024, 12
  • [6] Vibrio parahaemolyticus alters the community composition and function of intestinal microbiota in Pacific white shrimp, Penaeus vannamei
    Zhang, Xue
    Sun, Jingfeng
    Han, Zhuoran
    Chen, Feng
    Lv, Aijun
    Hu, Xiucai
    Sun, Xueliang
    Qi, Hongli
    Guo, Yongjun
    [J]. AQUACULTURE, 2021, 544
  • [7] CORRELATION BETWEEN INTESTINAL MICROBIOTA AND GROWTH OF WHITE SHRIMP (LITOPENAEUS VANNAMEI)
    Wu, J. Y.
    Yan, M. C.
    Sang, Y.
    Li, F.
    Luo, K.
    Hu, L. H.
    [J]. APPLIED ECOLOGY AND ENVIRONMENTAL RESEARCH, 2021, 19 (06): : 4993 - 5005
  • [8] Growth Performance and Intestinal Microbiota Diversity in Pacific White Shrimp Litopenaeus vannamei Fed with a Probiotic Bacterium, Honey Prebiotic, and Synbiotic
    Wahid Hasyimi
    Widanarni Widanarni
    Munti Yuhana
    [J]. Current Microbiology, 2020, 77 : 2982 - 2990
  • [9] Effect of Heat Treatment at Different Stages of Rigor on the Quality of Pacific White Shrimp (Litopenaeus vannamei)
    Bhat, Tariq Hussain
    Chouksey, Mithilesh Kumar
    Balange, Amjad Khansaheb
    Nayak, Binaya Bhusan
    [J]. JOURNAL OF AQUATIC FOOD PRODUCT TECHNOLOGY, 2017, 26 (07) : 770 - 780
  • [10] Individual and combined effects of ammonia-N and sulfide on the immune function and intestinal microbiota of Pacific white shrimp Litopenaeus vannamei
    Jiang, Li
    Feng, Jianxiang
    Ying, Rui
    Yin, Fangmin
    Pei, Surui
    Lu, Jianguo
    Cao, Yiting
    Guo, Jianlin
    Li, Zufu
    [J]. FISH & SHELLFISH IMMUNOLOGY, 2019, 92 : 230 - 240