Development and Validation of an HPLC-MS/MS Analytical Method for Quantitative Determination of Acetylsalicylic Acid and its Metabolite Salicylic Acid in Human Blood Plasma

被引：1
|
作者：
Tserkovnaya, K. M. [1 ]
Flisyuk, E., V [1 ]
Shabrov, V. N. [2 ]
机构：
[1] St Petersburg State Chem & Pharmaceut Univ, 14A Prof Popova St, Russia 197376, Russia
[2] LLC Quinta Analyt Yaroslavl, 52G Leningradskii Prosp, Yaroslavl 150045, Russia
来源：
PHARMACEUTICAL CHEMISTRY JOURNAL | 2022年 / 55卷 / 12期
关键词：
acetylsalicylic acid; human blood plasma; HPLC; MS; validation; bioequivalence;
D O I：
10.1007/s11094-022-02588-0
中图分类号：
R914 [药物化学];
学科分类号：
100701 ;
摘要：
An HPLC-MS/MS analytical method for quantitative determination of acetylsalicylic acid (ASA) as an antithrombotic agent and its metabolite salicylic acid (SA) in human blood plasma was developed and validated. The optimum concentration ranges were 0.020 - 1.200 mu g/mL for ASA and 0.200 - 12.00 mu g/mL for SA. The optimum conditions for sample preparation using liquid-liquid extraction by EtOAc followed by removal of the solvent in a stream of N-2, redissolution of the dry residue (to concentrate the sample and increase the method sensitivity), chromatography, and mass-spectrometric detection were selected. The developed method could be used further for pharmacokinetic and bioequivalence studies.
引用
收藏
页码：1401 / 1406
页数：6
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