Thermophilic strain bacillus species AA contains several site-specific endonucleases

被引：0
|
作者：
Alzhanov, DT
Alzhanova, DV
Zheleznaya, LA
Matvienko, NI [1 ]
机构：
[1] Russian Acad Sci, Inst Prot Res, Pushchino 142292, Moscow Region, Russia
[2] Russian Acad Sci, Inst Theoret & Expt Biophys, Pushchino 142292, Moscow Region, Russia
来源：
BIOCHEMISTRY-MOSCOW | 1998年 / 63卷 / 05期
关键词：
site-specific endonuclease; isoschizomer;
D O I：
暂无
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Thermophilic strain Bacillus species AA contains several site-specific endonucleases and three of them have been identified. BspAAI recognizes the sequence 5'-C down arrow TCGAG-3' and cleaves it after the first "C" forming 4-nucleotide 5'-ends and is an isoschizomer of XhoI. BspAAII recognizes the sequence 5'T down arrow CTAGA-3' and cleaves it after the first "T" forming 4-nucleotide 5'-ends and is an isoschizomer of XbaI. BspAAIII recognizes the sequence 5'-GGATCC-3' and is an isomer of BamHI. The optimal temperature and pH values are 42-48 degrees C and 7.0-8.0, respectively.
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页码：537 / 545
页数：9
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