BIOLOGICAL ACTIVITIES AND CHEMICAL COMPOSITION OF LICHENS FROM SERBIA

被引:0
|
作者
Kosanic, Marijana [1 ]
Rankovic, Branislav [1 ]
Stanojkovic, Tatjana [2 ]
Vasiljevic, Perica [3 ]
Manojlovic, Nedeljko [4 ]
机构
[1] Univ Kragujevac, Fac Sci, Dept Biol, Kragujevac 34000, Serbia
[2] Inst Oncol & Radiol Serbia, Belgrade 11000, Serbia
[3] Univ Nis, Fac Sci & Math, Dept Biol & Ecol, Nish 18000, Serbia
[4] Univ Kragujevac, Fac Med Sci, Dept Pharm, Kragujevac 34000, Serbia
来源
EXCLI JOURNAL | 2014年 / 13卷
关键词
HPLC analysis; biological activities; lichens; lichens compound; ANTIOXIDANT; EXTRACTS; METABOLITES; GROWTH; ASSAY;
D O I
暂无
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
The aim of this study is to investigate chemical composition of acetone extracts of the lichens Parmelia arseneana and Acarospora fuscata and in vitro antioxidant, antimicrobial, and anticancer activities of these extracts and gyrophoric acid isolated from A. fuscata. The HPLC-UV method was used for the identification of secondary metabolites. Stictic acid, norstictic acid, gyrophoric acid, usnic acid, atranorin and chloroatranorin were identified in the A. fuscata. In P. arseneana, we detected stictic acid, norstictic acid, usnic acid and atranorin, while gyrophoric acid was not identified. Antioxidant activity was evaluated by measuring the scavenging capacity of tested samples on DPPH and superoxide anion radicals, reducing the power of samples and determination of total phenolic compounds in extracts. As a result of the study, gyrophoric acid was found to have the largest DPPH radical scavenging activity with an IC50 value of 105.75 mu g/ml. Moreover, the tested samples had an effective superoxide anion radical scavenging and reducing power. The total content of phenol in extracts was determined as pyrocatechol equivalent. The antimicrobial activity was estimated by determination of the minimal inhibitory concentration by the broth microdilution method. The most active was also gyrophoric acid, with minimum inhibitory concentration values ranging from 0.019 to 1.25 mg/ml. Anticancer activity was tested against LS174 (human colon carcinoma cell line), A549 (human lung carcinoma cell line), Fem-x (malignant melanoma cell line), and a chronic myelogeneous leukaemia K562 cell line using the MTT method. Extract of P. arseneana expressed the strongest anticancer activity against all cell lines with IC50 values ranging from 11.61 to 47.06 mu g/ml.
引用
收藏
页码:1226 / 1238
页数:13
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