Determination of Sugars in Molasses by HPLC Following Solid-Phase Extraction

被引：44
|
作者：
Xu, Wanxia [1 ]
Liang, Lei [2 ]
Zhu, Mingjun [1 ,3 ]
机构：
[1] S China Univ Technol, Sch Biosci & Bioengn, Guangzhou Higher Educ Mega Ctr, Guangzhou 510006, Guangdong, Peoples R China
[2] Guangzhou Sugarcane Ind Res Inst, Guangdong Key Lab Sugarcane Improvement & Biorefi, Guangzhou, Guangdong, Peoples R China
[3] S China Univ Technol, State Key Lab Pulp & Paper Engn, Guangzhou 510006, Guangdong, Peoples R China
来源：
INTERNATIONAL JOURNAL OF FOOD PROPERTIES | 2015年 / 18卷 / 03期
基金：
中国国家自然科学基金;
关键词：
Solid-phase extraction; High performance liquid chromatography; Sugarcane molasses; Sugars; Internal standard; PERFORMANCE LIQUID-CHROMATOGRAPHY; LIGHT-SCATTERING DETECTION; INTERNAL STANDARD; OLIGOSACCHARIDES; VALIDATION; PROFILES; COLUMN; HONEY;
D O I：
10.1080/10942912.2013.837064
中图分类号：
TS2 [食品工业];
学科分类号：
0832 ;
摘要：
Fructose, glucose, and sucrose in sugarcane molasses are determined simultaneously by high performance liquid chromatography using maltose as internal standard with refractive index detector. The mixture of the diluted samples and internal standard was purified with Sep-Pak C-18 solid-phase extraction and filtered through a 0.22-mu m membrane before injection. The results showed that the linear ranges for fructose, glucose, and sucrose were 3.30-16.48, 1.80-9.02, and 5.94-29.70 g/L with the squared correlation coefficients (R-2) being 0.9986, 0.9987, and 0.9955, respectively. The method is simple, quantified, and time-saving for determination of sugars in sugarcane molasses.
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页码：547 / 557
页数：11
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