Breast cancer suppressor candidate 1 overexpression inhibited the proliferation, invasion and migration of SKOV3 cells

Objective: To investigate the effect of breast cancer suppressor candidate 1 (BCSC-1) overexpression on the biological function of SKOV3 ovarian carcinoma (OC) cells. Methods: Three groups: BCSC-1 group: SKOV3 cells transfected with pcDNA-BCSC-1; empty vector group: cells transfected with pcDNA vector; blank group: cells without any treatment. The effect of BCSC-1 overexpression on the proliferation, invasion, apoptosis, adhesion and migration of SKOV3 cells was examined by MTT, Transwell assay, flow cytometry, in-vitro cell adhesion assay and scratch assay, respectively. Tumor models in BAL B/C nude mice were established by injecting untransfected SKOV3 cells, cells transfected with empty plasmid or pcDNA-BCSC-1. Results: After transfection, the mRNA level of BCSC-1 in SKOV3 cells was significantly higher than that of empty vector group and untransfected group (P < 0.05). Compared with empty vector group, the growth rate of SKOV3 cells in BCSC-1 group was obviously slower (P < 0.05), the invasion rate, adhesion rate and migration distance was decreased significantly, while the apoptosis rate was increased evidently (P < 0.05). The growth rate in BCSC-1 group was evidently slower than that of empty vector (P < 0.05). No significant difference was detected between blank group and empty vector group. Conclusion: BCSC-1 overexpression can inhibit the proliferation, invasive, adhesive and migratory ability of SKOV3 cells.