A total of 350 nymphs of the common tick Ixodes ricinus (Linnaeus, 1758) were collected in an endemic focus of Lyme borreliosis (South Moravia, Czech Republic) and examined for the presence of the protozoan Babesia microti (Franca, 1909) by polymerase chain reaction (PCR), using primers specific for the B. microti gene encoding small subunit rRNA. The assay revealed five positive pools (out of 70 pools examined); the corresponding prevalence rate was about 1.5%. Sequence analysis of the PCR products confirmed their 100% homology with that of B. microti. The study represents the first evidence of B. microti in ixodid ticks in the Czech Republic.
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Washington State Univ, Sch Biol Sci, Pullman, WA 99164 USA
Cary Inst Ecosyst Studies, Millbrook, NY 12545 USAWashington State Univ, Sch Biol Sci, Pullman, WA 99164 USA
Brunner, Jesse L.
Killilea, Mary
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NYU, Dept Biol, New York, NY 10003 USAWashington State Univ, Sch Biol Sci, Pullman, WA 99164 USA
Killilea, Mary
Ostfeld, Richard S.
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Cary Inst Ecosyst Studies, Millbrook, NY 12545 USAWashington State Univ, Sch Biol Sci, Pullman, WA 99164 USA
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Natl Inst Res Dev Microbiol & Immunol Cantacuzino, Bucharest 050096, RomaniaNatl Inst Res Dev Microbiol & Immunol Cantacuzino, Bucharest 050096, Romania
Coipan, Elena Claudia
Vladimirescu, Alexandru Filip
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Natl Inst Res Dev Microbiol & Immunol Cantacuzino, Bucharest 050096, RomaniaNatl Inst Res Dev Microbiol & Immunol Cantacuzino, Bucharest 050096, Romania