Babesia microti (Piroplasmida:Babesiidae) in nymphal Ixodes ricinus (Acari:Ixodidae) in the Czech Republic

被引:18
|
作者
Rudolf, I
Golovchenko, M
Sikutová, S
Rudenko, N
Grubhoffer, L
Hubálek, Z
机构
[1] Acad Sci Czech Republ, Inst Vertebrate Biol, Brno 60365, Czech Republic
[2] Masaryk Univ, Fac Sci, Brno 60200, Czech Republic
[3] Acad Sci Czech Republ, Inst Parasitol, Ceske Budejovice 37005, Czech Republic
[4] Univ S Bohemia, Fac Biol Sci, Ceske Budejovice 37005, Czech Republic
关键词
D O I
10.14411/fp.2005.036
中图分类号
R38 [医学寄生虫学]; Q [生物科学];
学科分类号
07 ; 0710 ; 09 ; 100103 ;
摘要
A total of 350 nymphs of the common tick Ixodes ricinus (Linnaeus, 1758) were collected in an endemic focus of Lyme borreliosis (South Moravia, Czech Republic) and examined for the presence of the protozoan Babesia microti (Franca, 1909) by polymerase chain reaction (PCR), using primers specific for the B. microti gene encoding small subunit rRNA. The assay revealed five positive pools (out of 70 pools examined); the corresponding prevalence rate was about 1.5%. Sequence analysis of the PCR products confirmed their 100% homology with that of B. microti. The study represents the first evidence of B. microti in ixodid ticks in the Czech Republic.
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页码:274 / 276
页数:3
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