Chagas Immunochromatographic Rapid Test in the Serological Diagnosis of Trypanosoma cruzi Infection in Wild and Domestic Canids

被引:5
|
作者
Rodrigues, Esthefany S. [1 ,2 ]
Santos, Gilbert Q. [3 ,4 ]
Silva, Marlon Vicente da [1 ]
Barros, Juliana H. S. [1 ]
Bernardo, Aline R. [5 ]
Diniz, Rafaela L. [5 ]
Rubim, Nara M. [5 ]
Roque, Andre L. R. [1 ,6 ]
Jansen, Ana Maria [1 ]
Silva, Edimilson D. [5 ]
Xavier, Samanta C. C. [1 ,6 ]
机构
[1] Oswaldo Cruz Fdn FIOCRUZ, Oswaldo Cruz Inst, Lab Tripanosomatid Biol, Rio De Janeiro, Brazil
[2] Oswaldo Cruz Fdn FIOCRUZ, Natl Council Sci & Technol Dev CNPq, Inst Program Initiat Scholarships Technol Dev & I, Oswaldo Cruz Inst, Rio De Janeiro, Brazil
[3] Army Complementary Training Sch, Pedag Coordinat Sect, Salvador, BA, Brazil
[4] Salvador Mil Coll, EsFCEx, Salvador, BA, Brazil
[5] Oswaldo Cruz Fdn FIOCRUZ, Immunobiol Technol Inst Biomanguinhos, Diagnost Technol Lab, Rio De Janeiro, Brazil
[6] Fundacao Oswaldo Cruz, FIOCRUZ, Chagas Dis Translat Res Program, Fiochagas, Rio De Janeiro, Brazil
关键词
Trypanosoma cruzi; domestic and wild canids; dead-end and sentinel hosts; quimeric antigens; rapid test; PROTEIN-A; STAPHYLOCOCCUS-AUREUS; DISEASE; BINDING; AREAS; ANTIGENS; TRANSMISSION; IMMUNOASSAY; PERFORMANCE; MAMMALS;
D O I
10.3389/fcimb.2022.835383
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Canis lupus familiaris (domestic dog) represents a reliable sentinel for the occurrence of a well-established transmission cycle of Trypanosoma cruzi among wild mammals in the surroundings and, consequently, where the risk of human infection exists. Serological diagnosis is the chosen method to identify T. cruzi infection in dogs that, in Brazil, rarely present positive parasitological tests. The use of recombinant chimeric parasitic antigens results in a sensitive and specific serological diagnostic test in contrast to the use of crude T. cruzi antigens. Our objective was to evaluate the Chagas/Bio-Manguinhos Lateral Flow Immunochromatographic Rapid Test (Chagas-LFRT) for the diagnosis of T. cruzi infection in domestic dogs and the potential of application of this diagnostic platform to wild canid species. Two recombinant proteins (IBMP-8.1 and IBMP-8.4) that displayed the best performance in the enzyme immunoassay (ELISA) in previous studies were tested in a platform with two diagnostic bands. A panel of 281 dog serum samples was evaluated: 133 positive for T. cruzi by serological diagnosis, including 20 samples with positive blood cultures belonging to different discrete typing units (DTUs); 129 negative samples; and 19 samples from dogs infected by other trypanosomatids: Leishmania infantum, Trypanosoma rangeli, Trypanosoma caninum and Crithidia mellificae, in addition to samples infected by Anaplasma platys, Dirofilaria immitis and Erlichia sp. that were employed to evaluate eventual cross-reactions. We also evaluated the Chagas-LFRT to detect T. cruzi infection in 9 serum samples from six wild canid species. We observed that the intensity pattern of the bands was directly proportional to the serological titer observed in IFAT. The sensitivity was 94%, the specificity was 91% according to the ROC curve, and the defined cutoff was an optical density of 4.8. The agreement obtained was considered substantial by the kappa analysis (84%). From T. cruzi positive hemoculture samples, 88.9% were positive by Chagas-LFRT. The test was efficient in recognizing infections by five of the six T. cruzi DTUs. Cross-reactions were not observed in infections by L. infantum, T. rangeli, T. caninum and D. immitis; however, they were observed in sera of dogs infected by Crithidia mellificae, Anaplasma sp. and Erlichia sp. A strong reaction was observed when serum samples from wild canids were submitted to the Protein A affinity test, confirming its applicability for these species. This test will allow rapid preventive actions in areas with high risk to the emergence of Chagas disease in a safer, reliable, low-cost and immediate manner, without the need for more complex laboratory tests.
引用
收藏
页数:14
相关论文
共 50 条
  • [21] Performance evaluation of enzyme linked immunosorbent assay and lineblot for serological diagnosis of Chagas (Trypanosoma cruzi) disease
    Latz, A.
    Llano, M.
    Pavia, P.
    Puerta, C.
    Florez, A. C.
    Cucunuba, Z.
    Cuellar, A.
    Gonzalez, J.
    Licda, A. R.
    Roche, I.
    Zamora, D. M. B.
    [J]. TROPICAL MEDICINE & INTERNATIONAL HEALTH, 2013, 18 : 131 - 131
  • [22] Serological diagnosis of Chagas' disease: A potential confirmatory assay using preserved protein antigens of Trypanosoma cruzi
    Mendes, RP
    HoshinoShimizu, S
    DaSilva, AMM
    Mota, I
    Heredia, RAG
    Luquetti, AO
    Leser, PG
    [J]. JOURNAL OF CLINICAL MICROBIOLOGY, 1997, 35 (07) : 1829 - 1834
  • [23] Diagnosis of Acute Chagas Disease in a Belizean Child with Evidence of a Multiclonal Trypanosoma cruzi Infection
    Murray, Kristy O.
    Saldana, Miguel A.
    Gunter, Sarah M.
    Manzanero, Russell
    Zielinski-Gutierrez, Emily
    Herrera, Claudia
    Thompson, Julie M.
    Maliga, Adrianna
    Bautista, Kim
    Lino, Allison
    Hawes, Ella
    Ronca, Shannon E.
    Morey, Francis
    Fuentes, Rafael Chacon
    Lopez, Beatriz
    Dumonteil, Eric
    Morazan, Gerhaldine H.
    [J]. AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE, 2022, 107 (05): : 992 - 995
  • [24] Comparison of two immunochromatographic assays and the indirect immunofluorscence antibody test for diagnosis of Trypanosoma cruzi infection in dogs in south central Louisiana
    Nieto, Prixia D.
    Boughton, Roger
    Dorn, Patricia L.
    Steurer, Frank
    Raychaudhuri, Syamal
    Esfandiari, Javan
    Goncalves, Edson
    Diaz, James
    Malone, John B.
    [J]. VETERINARY PARASITOLOGY, 2009, 165 (3-4) : 241 - 247
  • [25] <it>TRYPANOSOMA CRUZI</it> IN DOMESTIC AND WILD RESERVOIRS OF THE DEPARTMENT OF CORDOBA
    Tovar Acero, Catalina
    Ricardo Caldera, Dina
    Espitia Perez, Lyda
    Chacon, Julio
    Doria, Miguel
    [J]. AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE, 2018, 99 (04): : 624 - 624
  • [26] Diagnostic Efficacy of Rapid Immunochromatographic Test in Diagnosis of Dengue Infection
    Kaur, Charanjeev
    Sharma, Sarbjeet
    [J]. JOURNAL OF PURE AND APPLIED MICROBIOLOGY, 2022, 16 (01): : 448 - 452
  • [27] Evaluation of a rapid immunochromatographic test for diagnosis of dengue virus infection
    Vaughn, DW
    Nisalak, A
    Kalayanarooj, S
    Solomon, T
    Dung, NM
    Cuzzubbo, A
    Devine, PL
    [J]. JOURNAL OF CLINICAL MICROBIOLOGY, 1998, 36 (01) : 234 - 238
  • [28] Indirect ELISA for the serological diagnosis of visceral leishmaniasis in wild canids
    Ferreira, Paulo R. B.
    Larangeira, Daniela F.
    de Oliveira, Lidia S.
    Malta, Marcelo de C. C.
    Gomes, Marta C.
    Bastos, Bruno L.
    Portela, Ricardo W.
    Barrouin-Melo, Stella Maria
    [J]. PESQUISA VETERINARIA BRASILEIRA, 2013, 33 (04): : 528 - 534
  • [29] A Highly Sensitive Rapid Diagnostic Test for Chagas Disease That Utilizes a Recombinant Trypanosoma cruzi Antigen
    Barfield, C. A.
    Barney, R. S.
    Crudder, C. H.
    Wilmoth, J. L.
    Stevens, D. S.
    Mora-Garcia, S.
    Yanovsky, M. J.
    Weigl, B. H.
    Yanovsky, J.
    [J]. IEEE TRANSACTIONS ON BIOMEDICAL ENGINEERING, 2011, 58 (03) : 814 - 817
  • [30] An immunochromatographic test for serological diagnosis of scrub typhus
    Yan, Shuhao
    Lu, Qingyu
    Tao, Qingyuan
    Lu, Yawei
    Gao, Bao
    Wang, Sibo
    Cai, Xusheng
    Ai, Lele
    Xiong, Xiaohui
    Cao, Min
    Tan, Weilong
    [J]. JOURNAL OF IMMUNOLOGICAL METHODS, 2024, 528