Functional differentiation of human pluripotent stem cells on a chip

被引：0
|
作者：
Giobbe, Giovanni G. [1 ,2 ]
Michielin, Federica [1 ,2 ]
Luni, Camilla [1 ,2 ]
Giulitti, Stefano [1 ,2 ]
Martewicz, Sebastian [1 ,2 ]
Dupont, Sirio [3 ]
Floreani, Annarosa [4 ]
Elvassore, Nicola [1 ,2 ]
机构：
[1] Univ Padua, Dept Ind Engn, Padua, Italy
[2] Venetian Inst Mol Med, Padua, Italy
[3] Univ Padua, Dept Mol Med, Padua, Italy
[4] Univ Padua, Dept Surg Oncol & Gastroenterol, Padua, Italy
来源：
NATURE METHODS | 2015年 / 12卷 / 07期
关键词：
ON-A-CHIP; EFFICIENT DIFFERENTIATION; DYNAMICS; CULTURE; TISSUES;
D O I：
10.1038/NMETH.3411
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Microengineering human "organs-on-chips" remains an open challenge. Here, we describe a robust microfluidics-based approach for the differentiation of human pluripotent stem cells directly on a chip. Extrinsic signal modulation, achieved through optimal frequency of medium delivery, can be used as a parameter for improved germ layer specification and cell differentiation. Human cardiomyocytes and hepatocytes derived on chips showed functional phenotypes and responses to temporally defined drug treatments.
引用
收藏
页码：637 / +
页数：7
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