Functional differentiation of human pluripotent stem cells on a chip

被引：0

作者：

Giobbe, Giovanni G. ^{[1
,2
]}

Michielin, Federica ^{[1
,2
]}

Luni, Camilla ^{[1
,2
]}

Giulitti, Stefano ^{[1
,2
]}

Martewicz, Sebastian ^{[1
,2
]}

Dupont, Sirio ^{[3
]}

Floreani, Annarosa ^{[4
]}

Elvassore, Nicola ^{[1
,2
]}

机构：

[1] Univ Padua, Dept Ind Engn, Padua, Italy

[2] Venetian Inst Mol Med, Padua, Italy

[3] Univ Padua, Dept Mol Med, Padua, Italy

[4] Univ Padua, Dept Surg Oncol & Gastroenterol, Padua, Italy

来源：

NATURE METHODS | 2015年 / 12卷 / 07期

关键词：

ON-A-CHIP; EFFICIENT DIFFERENTIATION; DYNAMICS; CULTURE; TISSUES;

D O I：

10.1038/NMETH.3411

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Microengineering human "organs-on-chips" remains an open challenge. Here, we describe a robust microfluidics-based approach for the differentiation of human pluripotent stem cells directly on a chip. Extrinsic signal modulation, achieved through optimal frequency of medium delivery, can be used as a parameter for improved germ layer specification and cell differentiation. Human cardiomyocytes and hepatocytes derived on chips showed functional phenotypes and responses to temporally defined drug treatments.

引用

页码：637 / +

页数：7

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