Determination of 5-hydroxytryptamine in serum by electrochemiluminescence detection with the aid of capillary electrophoresis

被引：22
|
作者：
Hu, Yuefang [1 ,2 ]
Xu, Wei [2 ]
Li, Jianping [1 ,2 ]
Li, Lanjuan [2 ]
机构：
[1] Guilin Univ Technol, Coll Chem & Bioengn, Guilin 541004, Peoples R China
[2] Zhejiang Univ, Sch Med, Affiliated Hosp 1, State Key Lab Diag & Treatment Infect Dis, Hangzhou 310003, Zhejiang, Peoples R China
来源：
LUMINESCENCE | 2012年 / 27卷 / 01期
关键词：
5-hydroxytryptamine; electrochemiluminescence; capillary electrophoresis; Tris(2; 2'-bipyridyl) ruthenium(II); SEROTONIN; NOREPINEPHRINE; CATECHOLAMINES; DOPAMINE; TISSUE;
D O I：
10.1002/bio.1326
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
A rapid, sensitive and simple electrochemiluminescence method for the determination of 5-hydroxytryptamine (5-HT) using capillary electrophoresis was proposed. The experimental parameters, including the detection potential, the concentration of Ru(bpy)32+, the concentration and pH of phosphate buffer for separation and detection, the injection voltage and time and the separation voltage on the determination of 5-HT, were optimized. Under the optimized conditions, the linear concentration range for 5-HT was 3.5x 10(-9)-5.1 x 10(-3) mol/L, with a detection limit of 5 x 10(-10) mol/L. The relative standard deviations (RSDs) of the ECL intensity and the migration times for six continuous injections of 1.0 mu mol/L 5-HT were 2.48% and 1.3%, respectively. The method was successfully applied to 5-HT assay in samples of human serum in 5min and the extraction recoveries with spiked serum samples were over 94.4%. Copyright (C) 2011 John Wiley & Sons, Ltd.
引用
收藏
页码：63 / 68
页数：6
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