NADPH oxidase NOX4 is a glycolytic regulator through mROS-HIF1α axis in thyroid carcinomas

被引：33
|
作者：
Tang, Ping [1 ]
Dang, Hao [2 ]
Huang, Jie [3 ]
Xu, Tao [1 ]
Yuan, Ping [1 ]
Hu, Jun [1 ]
Sheng, Jian-feng [1 ]
机构：
[1] Third Hosp Mianyang, Sichuan Mental Hlth Ctr, Otorhinolaryngol Head & Neck Surg, 190 East Jiannan Rd, Mianyang 621000, Sichuan, Peoples R China
[2] Third Hosp Mianyang, Sichuan Mental Hlth Ctr, Dept Clin Lab, 190 East Jiannan Rd, Mianyang 621000, Sichuan, Peoples R China
[3] Yunnan Jiehui Biotech Ltd, 9 Daxi Rd, Kunming 650215, Yunnan, Peoples R China
来源：
SCIENTIFIC REPORTS | 2018年 / 8卷
关键词：
RENAL TUMORIGENESIS; HYPOXIA; EXPRESSION;
D O I：
10.1038/s41598-018-34154-8
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
The function of the NAD(P)H oxidases (NOXs) family member NOX4 is to generate reactive oxygen species (ROS), however, the molecular function of NOX4 has not been fully studied and waiting to be clarified. To elucidate the function of endogenous Nox4 in human thyroid carcinomas, papillomatosis thyroid cancer cells were used to study the cell growth by knocking down the expression of NOX4 and knocking out its functional partner p22phox/CYBA. As a result, the increasement of mitochondrial ROS(mROS) was abolished due to both knockdown of NOX4 and p22phox knockout in hypoxia, which destabilized HIF1 alpha. decreasing glycolysis and retarded cell growth. These data suggests that Nox4 is potent oncotarget due to its role in regulating glycolysis through mROS-HIF1 alpha pathway, thereby mediating proliferation in thyroid carcinomas.
引用
收藏
页数：9
相关论文
共 44 条
  • [31] RESVERATROL REDUCES ENDOTHELIAL OXIDATIVE STRESS BY MODULATING THE GENE EXPRESSION OF SUPEROXIDE DISMUTASE 1 (SOD1), GLUTATHIONE PEROXIDASE 1 (GPX1) AND NADPH OXIDASE SUBUNIT (NOX4)
    Spanier, G.
    Xu, H.
    Xia, N.
    Tobias, S.
    Deng, S.
    Wojnowski, L.
    Forstermann, U.
    Li, H.
    JOURNAL OF PHYSIOLOGY AND PHARMACOLOGY, 2009, 60 : 111 - 116
  • [32] Overactivation of the MEK/ERK Pathway in Liver Tumor Cells Confers Resistance to TGF-β-Induced Cell Death through Impairing Up-regulation of the NADPH Oxidase NOX4
    Caja, Laia
    Sancho, Patricia
    Bertran, Esther
    Iglesias-Serret, Daniel
    Gil, Joan
    Fabregat, Isabel
    CANCER RESEARCH, 2009, 69 (19) : 7595 - 7602
  • [33] Suppression of angiotensin II-activated NOX4/NADPH oxidase and mitochondrial dysfunction by preserving glucagon-like peptide-1 attenuates myocardial fibrosis and hypertension
    Banks, Trenton E.
    Rajapaksha, Maheshinie
    Zhang, Li-Hui
    Bai, Feng
    Wang, Ning-Ping
    Zhao, Zhi-Qing
    EUROPEAN JOURNAL OF PHARMACOLOGY, 2022, 927
  • [34] Protein-tyrosine phosphatase 1B (PTP1B), a target of cellular oxidative inhibition following insulin stimulation, interacts with the NADPH oxidase homolog Nox4
    Mahadev, Kalyankar
    Ruffolo, Salvatore
    Wu, Xiangdong
    Ouedraogo, Raogo
    Kennedy, Brian P.
    Goldstein, Barry J.
    DIABETES, 2006, 55 : A301 - A301
  • [35] NOX4 alleviates breast cancer cell aggressiveness by co-ordinating mitochondrial turnover through PGC1α/Drp1 axis
    Bhadane, Deepali
    Kamble, Dinisha
    Deval, Mangesh
    Das, Subhajit
    Sitasawad, Sandhya
    CELLULAR SIGNALLING, 2024, 115
  • [36] Inhibition of NADPH oxidase activity or suppression of Nox4 counteracts TGFbeta1-mediated activation of human dermal fibroblasts in vitro and attenuates experimentally induced skin fibrosis in vivo
    Dosoki, H.
    Stegemann, A.
    Taha, M.
    Schnittler, H.
    Luger, T.
    Schroeder, K.
    Distler, H.
    Kerkhoff, C.
    Boehm, M.
    EXPERIMENTAL DERMATOLOGY, 2016, 25 (03) : E14 - E14
  • [37] High glucose-induced activation of PKC epsilon decreases the expression of bone morphogenetic protein 4 (Bmp4) and NADPH oxidase 4 (Nox4) through MAPK-dependent pathway in cultured mouse mesangial cells
    Osawa, Norihisa
    Hayashi, Toshihide
    Uzu, Takashi
    Babazono, Tetsuya
    Babazono, Suya
    Iwamoto, Yasuhiko
    Kashiwagi, Atsunori
    Maeda, Shiro
    DIABETES, 2007, 56 : A200 - A200
  • [38] DRD4 (Dopamine D4 Receptor) Mitigate Abdominal Aortic Aneurysm via Decreasing P38 MAPK (mitogen-activated protein kinase)/NOX4 (NADPH Oxidase 4) Axis-Associated Oxidative Stress
    Liu, Xuesong
    Guo, Yansong
    Yang, Yuxue
    Qi, Chunlei
    Xiong, Ting
    Chen, Yue
    Wu, Gengze
    Zeng, Chunyu
    Wang, Daxin
    HYPERTENSION, 2021, 78 (02) : 294 - 307
  • [39] Hypoxia promotes the metastasis of pancreatic cancer through regulating NOX4/KDM5A-mediated histone methylation modification changes in a HIF1A-independent manner
    Hongzhen Li
    Chunyan Peng
    Chenhui Zhu
    Shuang Nie
    Xuetian Qian
    Zhao Shi
    Mengyue Shi
    Yan Liang
    Xiwei Ding
    Shu Zhang
    Bin Zhang
    Xihan Li
    Guifang Xu
    Ying Lv
    Lei Wang
    Helmut Friess
    Bo Kong
    Xiaoping Zou
    Shanshan Shen
    Clinical Epigenetics, 2021, 13
  • [40] Hypoxia promotes the metastasis of pancreatic cancer through regulating NOX4/KDM5A-mediated histone methylation modification changes in a HIF1A-independent manner
    Li, Hongzhen
    Peng, Chunyan
    Zhu, Chenhui
    Nie, Shuang
    Qian, Xuetian
    Shi, Zhao
    Shi, Mengyue
    Liang, Yan
    Ding, Xiwei
    Zhang, Shu
    Zhang, Bin
    Li, Xihan
    Xu, Guifang
    Lv, Ying
    Wang, Lei
    Friess, Helmut
    Kong, Bo
    Zou, Xiaoping
    Shen, Shanshan
    CLINICAL EPIGENETICS, 2021, 13 (01)
12345