Functionalized single-walled carbon nanohorns for electrochemical biosensing

被引:43
|
作者
Liu, Xiaoqing [1 ,2 ]
Li, Haijuan [1 ,2 ]
Wang, Fuan [1 ,2 ]
Zhu, Shuyun [1 ,2 ]
Wang, Yuling [1 ,2 ]
Xu, Guobao [1 ]
机构
[1] Chinese Acad Sci, Changchun Inst Appl Chem, State Key Lab Electroanalyt Chem, Changchun 130022, Jilin, Peoples R China
[2] Chinese Acad Sci, Grad Univ, Changchun 130022, Jilin, Peoples R China
来源
BIOSENSORS & BIOELECTRONICS | 2010年 / 25卷 / 10期
基金
中国国家自然科学基金;
关键词
Carbon nanohorns; Carbon nanotubes; Noncovalent functionalization; Biosensors; Heme proteins; PHOTOINDUCED ELECTRON-TRANSFER; LAYER-BY-LAYER; HYDROGEN-PEROXIDE; HEME-PROTEINS; ENZYME ELECTRODE; NANOTUBES; MYOGLOBIN; IMPURITIES; FILMS; ELECTROCATALYSIS;
D O I
10.1016/j.bios.2010.02.027
中图分类号
Q6 [生物物理学];
学科分类号
071011 ;
摘要
Single-walled carbon nanohorns (SWNHs), distinguished by their high purity and distinct structure, were noncovalently functionalized with poly(sodium 4-styrenesulfonate). The functionalized SWNHs were characterized by scanning electron microscopy, atomic force microscopy, ultraviolet-visible spectroscopy. Fourier transform infrared spectroscopy, Raman spectroscopy, and thermogravimetry. Heme protein myoglobin was adsorbed onto surface of functionalized SWNHs to prepare electrochemical biosensor. Surface assembly process and direct electrochemistry of immobilized myoglobin were investigated by electrochemical impedance spectroscopy and cyclic voltammetry, respectively. The proposed biosensor exhibited good electrocatalysis to the reduction of hydrogen peroxide. The response was linear over the range 3-350 mu M with a detection limit of 0.5 mu M. Good reproducibility and stability of the biosensor were obtained toward hydrogen peroxide detection. (C) 2010 Elsevier B.V. All rights reserved.
引用
收藏
页码:2194 / 2199
页数:6
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