The effect of fluid shear stress on fibroblasts and stem cells on plane and groove topographies

被引：11

作者：

Lei, Xing ^{[1
,2
]}

Liu, Bin ^{[1
]}

Wu, Hao ^{[1
]}

Wu, Xiao ^{[3
]}

Wang, Xiu-Li ^{[4
]}

Song, Yue ^{[1
]}

Zhang, Shuai-Shuai ^{[1
]}

Li, Jun-Qin ^{[1
]}

Bi, Long ^{[1
]}

Pei, Guo-Xian ^{[1
]}

机构：

[1] Fourth Mil Med Univ, Xijing Hosp, Dept Orthoped, 15 Changle West St, Xian 710032, Peoples R China

[2] Linyi Peoples Hosp, Dept Orthoped Surg, Linyi, Shandong, Peoples R China

[3] Tsinghua Univ, Sch Aerosp Engn, Dept Engn Mech, Beijing, Peoples R China

[4] Fudan Univ, Dept Macromol Sci, State Key Lab Mol Engn Polymers, Shanghai, Peoples R China

来源：

CELL ADHESION & MIGRATION | 2020年 / 14卷 / 01期

基金：

中国国家自然科学基金; 国家重点研发计划;

关键词：

Frequency; alignment; polarity; adhesion; differentiate; migration; TITANIUM; SURFACE; REORIENTATION; BIOMATERIALS; MODEL;

D O I：

10.1080/19336918.2020.1713532

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

In this study, we aimed to study the effect of fluid shear stress on fibroblasts and BMSCs on plane and groove topographies. The results showed that 0.6-Hz stress had the greatest influence on the alignment, polarity, migration and adhesion of fibroblasts on plane by increasing the expression of reoriented actin and vinculin; whereas 1.0-Hz stress promoted differentiation of fibroblasts into myofibroblasts by increasing Col-I and alpha-SMA expression. Interestingly, under the given frequency stress, the groove structure strengthened the above characteristics of fibroblasts beyond adhesion, and promoted differentiation of BMSCs into myofibroblasts. The above results indicate that 0.6 Hz may improve the implant-tissue sealing, while 1.0-Hz stress probably causes the disordered fiber deposition around implants.

引用

页码：12 / 23

页数：12

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