Background: Increased intra-abdominal pressure (IAP) is an adverse complication seen in critically ill, injured, and postoperative patients. IAP is estimated via the measurement of bladder pressure. Few studies have been performed to establish the actual relationship between IAP and bladder pressure. The purpose of this study was to confirm the association between intravesicular pressure and IAP and to determine the bladder volume that best approximates IAP, Methods: Thirty-seven patients undergoing laparoscopy had intravesicular pressures measured with bladder volumes of 0, 50, 100, 150, and 200 mi, at directly measured intra-abdominal pressures of 0, 5, 10, 15, 20, and 25 mm Hg, Correlation coefficients and differences were then determined. Results: Across the IAP range of 0 to 25 mm tig using all of the tested bladder volumes, the difference between IAP and intravesicular pressures (bias) was -3.8 +/- 0.29 mm Hg (95% confidence interval) and measurements were well correlated (R-2 = 0.68), Assessing all IAPs tested, a bladder volume of 0 mt demonstrated the lowest bias (-0.79 +/- 0.73 mm Hg). When considering only elevated IAPs (25 mm tig), a bladder volume of 50 mt revealed the lowest bias (-1.5 +/- 1.36 mm Hg), A bladder volume of 50 mt in patients with elevated IAP resulted in an intravesicular pressure 1 to 3 mm Hg higher than IAP (95% confidence interval). Conclusion: Intravesicular pressure closely approximates IAP, Instillation of 50 mt of liquid into the bladder improves the accuracy of the intravesicular pressure in measuring elevated IAPs.