Serological and molecular evaluation of toxoplasmosis in patient undergoing chemotherapy for malignancies in southeast of Iran

被引：3
|
作者：
Hashemi, Seyed-Mehdi [1 ]
Mahmoodi, Shirin [2 ,3 ]
Mehravaran, Ahmad [2 ,3 ]
Mirahmadi, Hadi [2 ,3 ]
Khabisi, Samaneh Abdolahi [2 ,3 ]
Alijani, Ebrahim [4 ]
Solgi, Rahmat [5 ,6 ]
机构：
[1] Zahedan Univ Med Sci, Ali Ebne Abitalelb Hosp, Dept Internal Med, Clin Immunol Res Ctr,Hematol & Med Oncol Ward, Zahedan, Iran
[2] Zahedan Univ Med Sci, Resistant TB Inst, Infect Dis & Trop Med Res Ctr, Zahedan, Iran
[3] Zahedan Univ Med Sci, Dept Parasitol & Mycol, Fac Med, Zahedan, Iran
[4] Zahedan Univ Med Sci, Clin Immunol Res Ctr, Zahedan, Iran
[5] Birjand Univ Med Sci, Infect Dis Res Ctr, Birjand, Iran
[6] Birjand Univ Med Sci, Cellular & Mol Ctr, Birjand, Iran
来源：
GENE REPORTS | 2021年 / 23卷
关键词：
Toxoplasma gondii; LAMP assay; Polymerase chain reaction; REAL-TIME PCR; ISOTHERMAL AMPLIFICATION LAMP; GONDII INFECTION; DIAGNOSIS; CANCER; ASSOCIATION; ANTIBODIES;
D O I：
10.1016/j.genrep.2021.101163
中图分类号：
Q3 [遗传学];
学科分类号：
071007 ; 090102 ;
摘要：
Toxoplasma gondii is zoonotic protozoa that spread throughout the world. The aim of current study was determination of the prevalence of T. gondii infection in patient undergoing chemotherapy for malignancies using ELISA and two different molecular tests including Nested-PCR and LAMP techniques. This study was carried out in Ali Ibn Aboutaleb hospital in Zahedan cities, southeast of Iran. Totally, 154 serum samples from patient were test for detection of IgG and IgM antibodies against T. gondii by ELISA test. All of the samples were also test by Loop amplification (LAMP) and Nested-PCR with RE gene. Totally, 39.4% of the sample sera from cases were positive for IgG against T. gondii. After using RE-Nested-PCR technique, 29.8% sample sera were positively. After using LAMP technique, 33.1% of the samples were found to be positive. Based on our result, the RE-LAMP technique was more effective than RE-nested-PCR method in the detection of T. gondii DNA.
引用
收藏
页数：4
相关论文
共 30 条
  • [21] Molecular and Serological Evaluation of Neospora caninum Infection in Dogs from a Rural Setting in Fars Province, Southern Iran
    Hariri, Morvarid
    Arefkhah, Nasir
    Ghorbani, Fariba
    Namavari, Mehdi
    Omidian, Mostafa
    Sarkari, Bahador
    [J]. IRANIAN JOURNAL OF PARASITOLOGY, 2021, 16 (01) : 146 - 150
  • [22] Development and evaluation of a population pharmacokinetic-pharmacodynamic model of darbepoetin alfa in patients with nonmyeloid malignancies undergoing multicycle chemotherapy
    Agoram, Balaji
    Heatherington, Anne C.
    Gastonguay, Marc R.
    [J]. AAPS JOURNAL, 2006, 8 (03): : E552 - E563
  • [23] Evaluation of oral health related quality of life in subjects diagnosed with head and neck malignancies undergoing chemotherapy, radiotherapy, and surgery
    Naidu, Giridhar S.
    Shukla, Stuti
    Nagi, Ravleen
    Jain, Supreet
    Makkad, Ramanpal Singh
    [J]. JOURNAL OF INDIAN ACADEMY OF ORAL MEDICINE AND RADIOLOGY, 2019, 31 (03) : 228 - 233
  • [24] Development and evaluation of a population pharmacokinetic-pharmacodynamic model of darbepoetin alfa in patients with nonmyeloid malignancies undergoing multicycle chemotherapy
    Balaji Agoram
    Anne C. Heatherington
    Marc R. Gastonguay
    [J]. The AAPS Journal, 8
  • [25] Microscopic and Molecular Investigation of Intestinal Microsporidia in HIV + /AIDS and Cancer Patients Undergoing Chemotherapy in Mazandaran Province, North of Iran
    Bentolhoda Ahmadi
    Shahabeddin Sarvi
    Shahrbanoo Keihanian
    Lotfollah Davoudi
    Ahmad Daryani
    Hamed Mirjalali
    Ehsan Ahmadpour
    Mitra Sadeghi
    Seyed Ali Shariatzadeh
    Saeid Shakeri
    Seyed Abdollah Hosseini
    [J]. Acta Parasitologica, 2023, 68 : 690 - 698
  • [26] Microscopic and Molecular Investigation of Intestinal Microsporidia in HIV plus /AIDS and Cancer Patients Undergoing Chemotherapy in Mazandaran Province, North of Iran
    Ahmadi, Bentolhoda
    Sarvi, Shahabeddin
    Keihanian, Shahrbanoo
    Davoudi, Lotfollah
    Daryani, Ahmad
    Mirjalali, Hamed
    Ahmadpour, Ehsan
    Sadeghi, Mitra
    Shariatzadeh, Seyed Ali
    Shakeri, Saeid
    Hosseini, Seyed Abdollah
    [J]. ACTA PARASITOLOGICA, 2023, 68 (3) : 690 - 698
  • [27] Molecular and serological evaluation of visceral leishmaniasis in domestic dogs and cats in Maragheh County, north-west of Iran, 2018-2021
    Soleimani, Ali
    Mohebali, Mehdi
    Gholizadeh, Saber
    Bozorgomid, Arezoo
    Shafiei, Reza
    Raeghi, Saber
    [J]. VETERINARY MEDICINE AND SCIENCE, 2022, 8 (05) : 1898 - 1903
  • [28] Molecular and serological evaluation of zoonotic visceral leishmaniasis in dogs in a rural area of Fars province, southern Iran, as a source of Leishmania infantum infection
    Najafi, Laleh
    Omidian, Mostafa
    Rezaei, Zahra
    Shahabi, Saeed
    Ghorbani, Fariba
    Arefkhah, Nasir
    Mohebali, Mehdi
    Zaraei, Zabiolla
    Sarkari, Bahador
    [J]. VETERINARY MEDICINE AND SCIENCE, 2021, 7 (04) : 1082 - 1089
  • [29] OVAR-PROTECT - Leuprorelinacetat for protection of ovarian failure, a prospective, multicenter evaluation of GnRH-treatment among women with gynaecological malignancies undergoing chemotherapy.
    Chekerov, R.
    Jantke, A.
    Braicu, I.
    Ruhmland, B.
    Pietzner, K.
    Nedkova, I.
    Yalcinkaya, I.
    Sehouli, J.
    Oskay-Oezcelik, G.
    [J]. INTERNATIONAL JOURNAL OF GYNECOLOGICAL CANCER, 2016, 26 : 944 - 944
  • [30] Patient-related factors in determining the emetic risk potential for patients undergoing chemotherapy: An evaluation of predictive models for the development of a risk assessment tool
    Aapro, M.
    Dielenseger, P.
    Dranitsaris, G.
    Jordan, K.
    Molassiotis, A.
    Roeland, E.
    Schwartzberg, L.
    Warr, D.
    [J]. EUROPEAN JOURNAL OF CANCER, 2015, 51 : S234 - S234
123