Molecular cloning, purification and characterization of two endo-1,4-beta-glucanases from Aspergillus oryzae KBN616

被引：46
|
作者：
Kitamoto, N
Go, M
Shibayama, T
Kimura, T
Kito, Y
Ohmiya, K
Tsukagoshi, N
机构：
[1] MIE UNIV,FAC BIORESOURCES,TSU,MIE 504,JAPAN
[2] NAGOYA UNIV,SCH AGR,DEPT APPL BIOL SCI,NAGOYA,AICHI 464,JAPAN
来源：
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY | 1996年 / 46卷 / 5-6期
关键词：
D O I：
10.1007/s002530050857
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Two endo-1,4-beta-glucanase genes, designated celA and celB, from a shoyu koji mold Aspergillus oryzae KBN616, were cloned and characterized. The celA gene comprised 877 bp with two introns. The CelA protein consisted of 239 amino acids and was assigned to the cellulase family H. The celB gene comprised 1248 bp with no introns. The CelB protein consisted of 416 amino acids and was assigned to the cellulase family C. Both genes were overexpressed under the promoter of the A. oryzae taka-amylase A gene for purification and enzymatic characterization of CelA and CelB. CelA had a molecular mass of 31 kDa, a pH optimum of 5.0 and temperature optimum of 55 degrees C. whereas CelB had a molecular mass of 53 kDa, a pH optimum of 4.0 and temperature optimum of 45 degrees C.
引用
收藏
页码：538 / 544
页数：7
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