(R)-α-Trifluoromethylalanine as a 19F NMR Probe for the Monitoring of Protease Digestion of Peptides

被引：6

作者：

Devillers, Emmanuelle ^{[1
,2
]}

Chelain, Evelyne ^{[1
,2
]}

Dalvit, Claudio ^{[3
,4
]}

Brigaud, Thierry ^{[1
,2
]}

Pytkowicz, Julien ^{[1
,2
]}

机构：

[1] CY Cergy Paris Univ, CNRS, BIOCIS, 5 Mail Gay Lussac, F-95031 Neuville Sur Oise, Cergy Pontoise, France

[2] Univ Paris Saclay, BIOCIS, CNRS, F-92290 Chatenay Malabry, France

[3] Univ Neuchatel, Fac Sci, Ave Bellevaux 51, CH-2000 Neuchatel, Switzerland

[4] Lavis, Trento, Italy

来源：

CHEMBIOCHEM | 2022年 / 23卷 / 01期

关键词：

FABS; NMR spectroscopy; pepsin; trifluoromethylated peptides; trypsin; PROTEOLYTIC STABILITY; PROLYL OLIGOPEPTIDASE; VERSATILE TOOL; LABEL; IDENTIFICATION; THERAPEUTICS; CHYMOTRYPSIN; FLUORINATION; EFFICIENT; DESIGN;

D O I：

10.1002/cbic.202100470

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Fluorinated non-natural amino acids are useful tools for improving the bioavailability of peptides but can also serve as fluorinated probes in F-19 NMR-based enzymatic assays. We report herein that the use of the non-natural alpha-quaternarized (R)-alpha-trifluoromethylalanine ((R)-alpha-TfmAla) provides convenient and accurate monitoring of trypsin proteolytic activity and increases resistance towards pepsin degradation.

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页数：5

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