Mapping the 3D orientation of piconewton integrin traction forces

被引：92

作者：

Brockman, Joshua M. ^{[1
,2
]}

Blanchard, Aaron T. ^{[1
,2
]}

Ma, Victor Pui-Yan ^{[3
]}

Derricotte, Wallace D. ^{[3
,5
]}

Zhang, Yun ^{[3
]}

Fay, Meredith E. ^{[1
,2
]}

Lam, Wilbur A. ^{[1
,2
]}

Evangelista, Francesco A. ^{[3
]}

Mattheyses, Alexa L. ^{[4
,5
,6
]}

Salaita, Khalid ^{[1
,2
,3
]}

机构：

[1] Georgia Inst Technol, Wallace H Coulter Dept Biomed Engn, Atlanta, GA 30332 USA

[2] Emory Univ, Atlanta, GA 30322 USA

[3] Emory Univ, Dept Chem, 1515 Pierce Dr, Atlanta, GA 30322 USA

[4] Emory Univ, Sch Med, Dept Cell Biol, Atlanta, GA 30322 USA

[5] Morehouse Coll, Dept Chem, Atlanta, GA USA

[6] Univ Alabama Birmingham, Dept Cell Dev & Integrat Biol, Birmingham, AL 35294 USA

来源：

NATURE METHODS | 2018年 / 15卷 / 02期

基金：

美国国家科学基金会;

关键词：

FLUORESCENCE MICROSCOPY; PLATELET ACTIVATION; FOCAL ADHESIONS; DENSITY; DYNAMICS; NANOSCALE; CELLS;

D O I：

10.1038/nmeth.4536

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Mechanical forces are integral to many biological processes; however, current techniques cannot map the magnitude and direction of piconewton molecular forces. Here, we describe molecular force microscopy, leveraging molecular tension probes and fluorescence polarization microscopy to measure the magnitude and 3D orientation of cellular forces. We mapped the orientation of integrin-based traction forces in mouse fibroblasts and human platelets, revealing alignment between the organization of force-bearing structures and their force orientations.

引用

页码：115 / +

页数：9

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