Preparation of extended metaphase chromosomes from human cultured cells using a topoisomerase II inhibitor, ICRF-193

被引：2
|
作者：
Kohda, A [1 ]
Taguchi, H [1 ]
Okumura, K [1 ]
机构：
[1] Mie Univ, Fac Bioresources, Mol & Cellular Biol Lab, Tsu, Mie 5148507, Japan
来源：
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | 2001年 / 65卷 / 05期
关键词：
chromosome preparations; fluorescence in situ hybridization; topoisomerase II inhibitor; HL60; HepG2;
D O I：
10.1271/bbb.65.1248
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Effects of ICRF-193, a topoisomerase II inhibitor, on metaphase chromosome preparations were examined. A short-time exposure of this drug to human HL60 cells in a suspension culture before harvest resulted in obtaining more extended metaphase chromosomes, The length, of chromosome 6 identified by fluorescence in situ hybridization was twice as long with this drug treatment. Together with effectiveness for adherent HepG2 cells, these results suggest that treatments with ICRF-193 provide a simple and reliable method for chromosome preparations from extended cultured metaphase cells.
引用
收藏
页码：1248 / 1251
页数：4
相关论文
共 31 条
  • [21] INHIBITION OF DNA TOPOISOMERASE-II BY ICRF-193 INDUCES POLYPLOIDIZATION BY UNCOUPLING CHROMOSOME DYNAMICS FROM OTHER CELL-CYCLE EVENTS
    ISHIDA, R
    SATO, M
    NARITA, T
    UTSUMI, KR
    NISHIMOTO, T
    MORITA, T
    NAGATA, H
    ANDOH, T
    JOURNAL OF CELL BIOLOGY, 1994, 126 (06): : 1341 - 1351
  • [22] ICRF-193, AN INHIBITOR OF TOPOISOMERASE-II, DEMONSTRATES THAT DNA-REPLICATION IN SPERM NUCLEI RECONSTITUTED IN XENOPUS EGG EXTRACTS DOES NOT REQUIRE CHROMATIN DECONDENSATION
    TAKASUGA, Y
    ANDOH, T
    YAMASHITA, J
    YAGURA, T
    EXPERIMENTAL CELL RESEARCH, 1995, 217 (02) : 378 - 384
  • [23] Hypersensitivity of Ku-deficient cells toward the DNA topoisomerase II inhibitor ICRF-193 suggests a novel role for Ku antigen during the G2 and m phases of the cell cycle
    Muñoz, P
    Zdzienicka, MZ
    Blanchard, JM
    Piette, J
    MOLECULAR AND CELLULAR BIOLOGY, 1998, 18 (10) : 5797 - 5808
  • [24] ICRF-193, AN INHIBITOR OF TOPOISOMERASE-II, DEMONSTRATES THAT DNA-REPLICATION IN SPERM NUCLEI RECONSTITUTED IN XENOPUS EGG EXTRACTS DOES NOT REQUIRE CHROMATIN DECONDENSATION (VOL 217, PG 378, 1995)
    TAKASUGA, Y
    ANDOH, T
    YAMASHITA, J
    YAGURA, T
    EXPERIMENTAL CELL RESEARCH, 1995, 220 (01) : 241 - 241
  • [25] The catalytic DNA topoisomerase II inhibitor ICRF-193 and all-trans retinoic acid cooperatively induce granulocytic differentiation of acute promyelocytic leukemia cells:: Candidate drugs for chemo-differentiation therapy against acute promyelocytic leukemia
    Niitsu, N
    Higashihara, M
    Honma, Y
    EXPERIMENTAL HEMATOLOGY, 2002, 30 (11) : 1273 - 1282
  • [26] Interaction of the DNA topoisomerase II catalytic inhibitor meso-2,3-bis(3,5-dioxopiperazine-1-yl)butane (ICRF-193), a bisdioxopiperazine derivative, with the conserved region(s) of eukaryotic but not prokaryotic enzyme
    Sato, M
    Ishida, R
    Narita, T
    Kato, JI
    Ikeda, H
    Fukazawa, H
    Andoh, T
    BIOCHEMICAL PHARMACOLOGY, 1997, 54 (05) : 545 - 550
  • [27] The catalytic DNA topoisomerase II inhibitor dexrazoxane (ICRF-187) induces differentiation and apoptosis in human leukemia K562 cells
    Hasinoff, BB
    Abram, ME
    Barnabé, N
    Khélifa, T
    Allan, WP
    Yalowich, JC
    MOLECULAR PHARMACOLOGY, 2001, 59 (03) : 453 - 461
  • [28] Selection of human leukemic CEM cells for resistance to the DNA topoisomerase II catalytic inhibitor ICRF-187 results in increased levels of topoisomerase IIα and altered G2/M checkpoint and apoptotic responses
    Morgan, SE
    Cadena, RS
    Raimondi, SC
    Beck, WT
    MOLECULAR PHARMACOLOGY, 2000, 57 (02) : 296 - 307
  • [29] Human small cell lung cancer NYH cells selected for resistance to the bisdioxopiperazine topoisomerase II catalytic inhibitor ICRF-187 demonstrate a functional R162Q mutation in the Walker A consensus ATP binding domain of the α isoform
    Wessel, I
    Jensen, LH
    Jensen, PB
    Falck, J
    Rose, A
    Roerth, M
    Nitiss, JL
    Sehested, M
    CANCER RESEARCH, 1999, 59 (14) : 3442 - 3450
  • [30] (S)-Erypoegin K, an isoflavone isolated from Erythrina poeppigiana, is a novel inhibitor of topoisomerase IIα: Induction of G2 phase arrest in human gastric cancer cells
    Hikita, Kiyomi
    Yamakage, Yuko
    Okunaga, Honoka
    Motoyama, Yui
    Matsuyama, Haruka
    Matsuoka, Kenta
    Murata, Tomiyasu
    Nakayoshi, Tomoki
    Oda, Akifumi
    Kato, Kuniki
    Tanaka, Hitoshi
    Asao, Naoki
    Dan, Shingo
    Kaneda, Norio
    BIOORGANIC & MEDICINAL CHEMISTRY, 2021, 30
1234