Thermostable DNA-polymerase from Thermus thermophilus B35:: Isolation and characterization of some properties

被引：0
|
作者：
Rechkunova, NI
Akishev, AG
Lebedeva, NA
Khodyreva, SN
Degtyarev, SK
Lavrik, OI
机构：
[1] Russian Acad Med Sci, Inst Mol Pathol & Ecol Biochem, Siberian Branch, Novosibirsk 630117, Russia
[2] Russian Acad Sci, Novosibirsk Bioorgan Chem Inst, Siberian Branch, Novosibirsk 630090, Russia
来源：
BIOCHEMISTRY-MOSCOW | 1998年 / 63卷 / 11期
关键词：
thermostable DNA-polymerase; isolation; properties; Thermus; DNA amplification;
D O I：
暂无
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Thermostable Tte DNA-polymerase was isolated from the strain Thermus thermophilusB35 which was found in hot spring water. The enzyme with molecular mass 87 kD was isolated using sequential chromatography on DEAE-Sepharose, hydroxylapatite, hexyl-agarose, and heparin-Sepharose. Biochemical properties of Tte DNA-polymerase are similar to those of Tth DNA-polymerase isolated from Thermus thermophilus HB8; however, practical application of Tte-Pol seems to be more favorable due to higher temperature optimum of this enzyme and lack of restriction endonucleases in the initial strain.
引用
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页码：1266 / 1270
页数：5
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