Concentration of Polyphenolic Compounds from Grape Seed by Nanofiltration Technology

In order to evaluate the applicability of nanofiltration (NF) polyphenols determined by total phenolic compounds content with application of Folin method in grape seed extract, response surface analysis methodology was used to analyze the concentration process with the indices of membrane fouling and antioxidant activity. In addition to the influencing factors of molecular weight cut-off (MWCO) of NF membrane, procyanidin concentration and pH value, the evaluation index of procyanidin rejection was taken into account for the process optimization by Box-Behnken response surface method on the basis of single factor test. According to Box-Behnken central composite experiment design, the optimal conditions were obtained as follows: NF MWCO of 400 Da, 27.66 mu g/mL procyanidins, and pH 5.20. The predicted rejection of procyanidins under the optimum conditions was 97.17% and the experimental value was 96.36 +/- 0.87%, which was in accordance with the predicted value. The experimental value of total polyphenolic content (TPC) was 91.09 +/- 0.46%. The antioxidant activity was increased about 2.24 times and the antioxidant activity was correlated with the procyanidin content. Moreover, it was easy to clean membrane fouling. The NF was an effective method for concentrating polyphenolic compounds from grape seed extracts without the loss of polyphenolic compounds. The agricultural product utilization was improved greatly and the power consumption was decreased by the NF technology.