A novel polymerase chain reaction assay for the detection and speciation of thermophilic Campylobacter spp.

被引：0
|
作者：
Jackson, CJ [1 ]
Fox, AJ [1 ]
Jones, DM [1 ]
机构：
[1] WITHINGTON HOSP,MANCHESTER PUBL HLTH LAB,DEPT MOL BIOL,MANCHESTER M20 2LR,LANCS,ENGLAND
来源：
JOURNAL OF APPLIED BACTERIOLOGY | 1996年 / 81卷 / 05期
关键词：
D O I：
暂无
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
A novel PCR amplification method is described which is specific for the thermophilic, enteropathogenic species Campylobacter jejuni, Camp. coli and Camp. upsaliensis. Rapid, accurate speciation of amplified strains is possible on the basis of restriction fragment length polymorphisms of PCR products digested with three restriction enzymes, AluI, DdeI and DraI. The sensitivity of detection is 25 cfu in water, and 2 x 10(3) cfu in full cream milk. An epidemiological application of the assay in detecting non-culturable campylobacters from a contaminated potable mater supply is described.
引用
收藏
页码：467 / 473
页数：7
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