Antiseptic Activity and Phenolic Constituents of the Aerial Parts of Vitex negundo var. cannabifolia

Four phenolics, salviaplebeiaside (1), gamma-tocopherol (2), chrysosplenol-D (4), and isovitexin (5), along with alpha-tocoquinone (3) and beta-sitosterol (6) were isolated from the aerial parts of Vitex negundo var. cannabifolia. The isolation was performed using bio-assay tracking experiments. The structures of compounds 1-5 were established by spectroscopic means. The antibacterial activities of the compounds were assessed against Escherichia coli, Bacillus subtilis, Micrococcus tetragenus and Pseudomonas fluorescens. Chrysosplenol-D (4) exhibited activities against all the four spoilage microorganisms.