Effect of insulin-like growth factor-1 (IGF-1) on the gluconeogenesis in calf hepatocytes cultured in vitro

被引：17
|
作者：
Wang, Jianguo [1 ]
Zhu, Xiaoyan [1 ]
Chen, Chengzhen [2 ]
Li, Xiaobing [1 ]
Gao, Yan [2 ]
Li, Peng [1 ,3 ]
Zhang, Yi [3 ]
Long, Miao [3 ]
Wang, Zhe [1 ]
Liu, Guowen [1 ]
机构：
[1] Jilin Univ, Coll Anim Sci & Vet Med, Changchun 130062, Peoples R China
[2] Jilin Univ, Lab Anim Ctr, Changchun 130062, Peoples R China
[3] Shenyang Agr Univ, Coll Anim Sci & Vet Med, Shenyang 110161, Peoples R China
来源：
MOLECULAR AND CELLULAR BIOCHEMISTRY | 2012年 / 362卷 / 1-2期
基金：
中国国家自然科学基金;
关键词：
IGF-1; PC; PEPCK; Hepatocyte; DAIRY-COWS; PHOSPHOENOLPYRUVATE CARBOXYKINASE; MESSENGER-RNA; TRANSITION; EXPRESSION; LACTATION; HORMONE; ENZYMES; LIVER;
D O I：
10.1007/s11010-011-1130-9
中图分类号：
Q2 [细胞生物学];
学科分类号：
071009 ; 090102 ;
摘要：
The major role of insulin-like growth factor-1 (IGF-1) in the liver is to mediate glucose uptake in hepatocytes to synthesize glycogen and maintain blood glucose homeostasis. In this study, to evaluate the role of IGF-1 on gluconeogenesis and nutrient metabolism in dairy cattle, pyruvate carboxylase (PC) and phosphoenolpyruvate carboxykinase (PEPCK) expression and enzyme activity were evaluated in primary cultures of bovine hepatocytes treated with different concentrations of IGF-1 by quantitative polymerase chain reaction and spectrophotometry, respectively. The results showed that expression of PC and PEPCK were significantly lower in bovine hepatocytes by IGF-1 treatment in test group compare to the control group (P < 0.01). As IGF-1 concentration increased, PC and PEPCK enzyme activity in bovine hepatocytes decreased. Evaluating PC and PEPCK mRNA levels and enzyme activity may thus be useful to monitor subclinical ketosis in dairy cows.
引用
收藏
页码：87 / 91
页数：5
相关论文
共 50 条
  • [31] ALCOHOL ALTERS INSULIN-LIKE GROWTH FACTOR-1 IGF-1 AND IGF-1/ESTRADIOL INDUCED TRANSFORMING GROWTH FACTOR β1 RELEASE FROM HYPOTHALAMIC ASTROCYTES
    Hiney, J. K.
    Srivastava, V. K.
    Dees, W. L.
    ALCOHOLISM-CLINICAL AND EXPERIMENTAL RESEARCH, 2012, 36 : 264A - 264A
  • [32] Do deficiencies in growth hormone and insulin-like growth factor-1 (IGF-1) shorten or prolong longevity?
    Laron, Z
    MECHANISMS OF AGEING AND DEVELOPMENT, 2005, 126 (02) : 305 - 307
  • [33] Growth hormone (GH) and insulin-like growth factor-1 (IGF-1) in the elderly: Clinical trial results
    Ceda, GP
    Dall'Aglio, E
    Salimbeni, I
    Rocci, A
    Corradi, F
    Mazzoni, S
    Greco, F
    Ridolo, E
    Ceresini, G
    Banchini, A
    Valenti, G
    Hoffman, AR
    ARCHIVES OF GERONTOLOGY AND GERIATRICS, 2002, : 81 - 92
  • [34] THE EFFECTS OF RECOMBINANT HUMAN IGF-1 (INSULIN-LIKE GROWTH FACTOR-1) INJECTION ON LIVER GROWTH IN RATS
    Kocamis, H.
    Sari, Karadag E.
    Aslan, S.
    Gulmez, N.
    Deprem, T.
    ACTA VETERINARIA-BEOGRAD, 2009, 59 (01): : 25 - 31
  • [35] Genetic and Physiological Effects of Insulin-Like Growth Factor-1 (IGF-1) on Human Urate Homeostasis
    Mount, David B.
    Leask, Megan P.
    Merriman, Tony
    Mandal, Asim
    JOURNAL OF THE AMERICAN SOCIETY OF NEPHROLOGY, 2022, 33 (11): : 145 - 145
  • [36] Genetic and Physiological Effects of Insulin-Like Growth Factor-1 (IGF-1) on Human Urate Homeostasis
    Mandal, Asim K.
    Leask, Megan P.
    Sumpter, Nicholas A.
    Choi, Hyon K.
    Merriman, Tony R.
    Mount, David B.
    JOURNAL OF THE AMERICAN SOCIETY OF NEPHROLOGY, 2023, 34 (03): : 451 - 466
  • [37] Intrauterine Growth Retardation (IUGR) as a Novel Condition of Insulin-Like Growth Factor-1 (IGF-1) Deficiency
    Martin-Estal, I.
    de la Garza, R. G.
    Castilla-Cortazar, I.
    REVIEWS OF PHYSIOLOGY, BIOCHEMISTRY AND PHARMACOLOGY, VOL 170, 2016, 170 : 1 - 35
  • [38] Serum insulin-like growth factor-1 in centenarians: Implications of IGF-1 as a rapid turnover protein
    Arai, Y
    Hirose, N
    Yamamura, K
    Shimizu, K
    Takayama, M
    Ebihara, Y
    Osono, Y
    JOURNALS OF GERONTOLOGY SERIES A-BIOLOGICAL SCIENCES AND MEDICAL SCIENCES, 2001, 56 (02): : M79 - M82
  • [39] The role of insulin-like growth factor-1 (IGF-1) in wound healing of human skin cells
    Chen, S.
    Conte, J.
    Yim, S.
    Ptchelintsev, D.
    Santhanam, U.
    JOURNAL OF INVESTIGATIVE DERMATOLOGY, 2009, 129 : S8 - S8
  • [40] The effect of subcutaneous Insulin-like Growth Factor-1 (IGF-1) injection on rabbit auricular cartilage autograft viability
    Beriat, Guclu Kaan
    Akmansu, Sefik Halit
    Dogan, Cem
    Ezerarslan, Hande
    Han, Unsal
    Saglam, Mehmet
    Senel, Oytun Okan
    Kocaturk, Sinan
    BOSNIAN JOURNAL OF BASIC MEDICAL SCIENCES, 2012, 12 (04) : 213 - 218
12345