Flammulin:: A novel ribosome-inactivating protein from fruiting bodies of the winter mushroom Flammulina velutipes

被引：39
|
作者：
Wang, HX
Ng, TB [1 ]
机构：
[1] Chinese Univ Hong Kong, Dept Biochem, Shatin, Hong Kong, Peoples R China
[2] China Agr Univ, Dept Microbiol, Beijing, Peoples R China
来源：
BIOCHEMISTRY AND CELL BIOLOGY-BIOCHIMIE ET BIOLOGIE CELLULAIRE | 2000年 / 78卷 / 06期
关键词：
mushroom; ribosome-inactivating protein; fruiting body;
D O I：
10.1139/bcb-78-6-699
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
A protein with a molecular weight of 40 kDa, capable of inhibiting cell-free translation in a rabbit reticulocyte lysate system with an IC50 of 0.25 nM, was isolated from fruiting bodies of the mushroom Flammulina velutipes. The protein, designated flammulin, was devoid of ribonuclease activity. Flammulin was unadsorbed on DEAE-cellulose at neutral pH and low ionic strength and adsorbed on CM-Sepharose and Affi-gel blue gel under similar conditions. Its N-terminal sequence demonstrates sites of similarity to those of plant ribosome-inactivating proteins (RIPs).
引用
收藏
页码：699 / 702
页数：4
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