A study of interaction between α-chymotrypsin and 18-crown-6 in organic solvents by Raman spectroscopy

被引：0
|
作者：
Brandt, NN
Sakodynskaya, IK
Chikishev, AY
机构：
[1] Moscow MV Lomonosov State Univ, Fac Phys, Ctr Int Laser, Moscow 119899, Russia
[2] Moscow MV Lomonosov State Univ, Fac Chem, Moscow 119899, Russia
来源：
RUSSIAN JOURNAL OF PHYSICAL CHEMISTRY | 2001年 / 75卷 / 06期
关键词：
D O I：
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中图分类号：
O64 [物理化学（理论化学）、化学物理学];
学科分类号：
070304 ; 081704 ;
摘要：
Conformational changes of alpha -chymotrypsin caused by its interactions with organic solvents and 18-crown-6 were studied by Raman spectroscopy. The IR spectra of a-chymotrypsin lyophilized in the presence and absence of 18-crown-6, in the solid state, in acetonitrile and cyclohexane, and at various relative enzyme and crown molar concentrations were recorded. Tris(hydroxymethyl)aminomethane and its complexes with 18-crown-6 were used as chemical models to study interaction between the amino groups of the protein and the crown. The conformation of enzyme molecules in water was found to be drastically different from that in the "dry" sample and in organic solvents. The influence of the crown and solvents on conformation-sensitive enzyme bands is discussed.
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页码：928 / 932
页数：5
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