A naphthalene-based fluorescent probe with a large Stokes shift for mitochondrial pH imaging

被引：33
|
作者：
Lin, Bo [1 ]
Fan, Li [2 ]
Ge, Jinyin [1 ]
Zhang, Wenjia [1 ]
Zhang, Caihong [1 ]
Dong, Chuan [2 ]
Shuang, Shaomin [1 ]
机构：
[1] Shanxi Univ, Coll Chem & Chem Engn, Taiyuan 030006, Shanxi, Peoples R China
[2] Shanxi Univ, Inst Environm Sci, Taiyuan 030006, Shanxi, Peoples R China
来源：
ANALYST | 2018年 / 143卷 / 20期
基金：
中国国家自然科学基金;
关键词：
LIVING CELLS; RATIOMETRIC DETECTION; CARDIAC MITOCHONDRIA; HYDROGEN-SULFIDE; PROTEIN MUTANT; BETA-CELLS; TISSUES; ACIDIFICATION; TRANSPORT; CALCIUM;
D O I：
10.1039/c8an01371c
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
A mitochondrial-targeted pH fluorescent probe 4-(2-(6-hydroxynaphthalen-2-yl)vinyl)-1-methylpyridin-1-ium was facilely synthesized via the carbon-carbon double bond bridging of 6-hydroxy-2-naphthaldehyde and 1,4-dimethylpyridinium iodide salt. The probe exhibited remarkable pH-dependent behavior in the linear range of 7.60-10.00, with a pK(a) value of 8.85 +/- 0.04 near mitochondrial pH. A significantly large Stokes shift of 196 nm was obtained, which reduces the interference of excitation light. Application of the probe in live HepG2 cells indicated that the probe had excellent mitochondrial targeting ability and was successfully used to visualize mitochondrial pH fluctuations in live cells.
引用
收藏
页码：5054 / 5060
页数：7
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