Profiles of HCV core protein and viremia in chronic hepatitis C: Possible protective role of core antigen in liver damage

被引:14
|
作者
Carabaich, A [1 ]
Ruvoletto, M [1 ]
Bernardinello, E [1 ]
Tono, N [1 ]
Cavalletto, L [1 ]
Chemello, L [1 ]
Gatta, A [1 ]
Pontisso, P [1 ]
机构
[1] Univ Padua, Dipartimento Med Clin & Sperimentale, Clin Med 5, I-35123 Padua, Italy
关键词
nucleocapsid particles; liver disease activity; density gradient analysis; lipid associated HCV particles;
D O I
10.1002/jmv.20322
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
The relation between HCV core antigen and HCV RNA has been confirmed in patients with chronic hepatitis C and a parallel behavior of the two markers has been described in early kinetics analysis during antiviral therapy. Variations of the core antigen to HCV RNA ratio have been reported in individual patients and the existence of nucleocapsid particles, not always associated with viral genomes, have been described. To assess the characteristics of HCV core antigen reactivity in relation to viremia in patients with different clinical profiles, 233 patients with chronic hepatitis C were studied serially. Group A included 54 asymptornatic HCV carriers, group B included 8 viremic patients with biochemical long-term response after antiviral therapy, while group C was composed of 171 patients with chronic liver disease and 75 were treated with combination therapy. Core antigen levels were not significantly different in the three groups of patients and a wide range of antigenic reactivity was observed in individual patients. A close relationship was observed between core antigen and HCV RNA, although their ratio was significantly higher in biochemical long-term responders (group 13), compared to the other groups (P < 0.05). Physicochemical characterization of core antigen reactivity by equilibrium CsCl density gradient identified two distinct peaks migrating at 1.08-1.12 g/ml CsCl density and at 1.18-1.31 CsCl density, respectively. The first one, corresponding to the lipid-associated fraction, contained higher amounts of core antigen reactivity and was associated with clinical remission of liver damage, while the second peak, corresponding to naked nucleocapsids, was observed mainly in sera with active disease. In conclusion, a close relationship between core and HCV RNA was documented both in treated and untreated patients. The finding of an excess of lipid-associated core particles in a subset of viremic patients without biochemical activity of liver disease suggests their protective effect in liver cell damage. (c) 2005 Wiley-Liss, Inc.
引用
收藏
页码:55 / 60
页数:6
相关论文
共 50 条
  • [31] EXPRESSION OF HCV-RELATED ANTIGEN AND LIVER-DAMAGE IN THE PATIENTS WITH CHRONIC HEPATITIS-C
    HIRAMATSU, N
    HAYASHI, N
    HARUNA, Y
    KASAHARA, A
    FUSAMOTO, H
    MORI, C
    FUKE, I
    OKAYAMA, H
    KAMADA, T
    HEPATOLOGY, 1992, 16 (02) : 544 - 544
  • [32] The role of PTEN - HCV core interaction in hepatitis C virus replication
    Wu, Qi
    Li, Zhubing
    Mellor, Paul
    Zhou, Yan
    Anderson, Deborah H.
    Liu, Qiang
    SCIENTIFIC REPORTS, 2017, 7
  • [33] The role of PTEN - HCV core interaction in hepatitis C virus replication
    Qi Wu
    Zhubing Li
    Paul Mellor
    Yan Zhou
    Deborah H. Anderson
    Qiang Liu
    Scientific Reports, 7
  • [34] The role of hepatitis C core antigen (HCV-cAg) testing in the era of hepatitis C direct-acting antivirals (DAAs)
    Paparello, J.
    Lee, M.
    Nebbia, G.
    O'Shea, S.
    Mullen, J.
    Lewis, C.
    Douthwait, S.
    Kulasegaram, R.
    HIV MEDICINE, 2017, 18 : 10 - 11
  • [35] Quantitative determination of hepatitis C virus core antigen (HCVAg) and prediction of response to therapy in chronic HCV infection
    Jacobs, R
    Brown, D
    Diment, J
    Whitehe, J
    Dusheiko, G
    JOURNAL OF HEPATOLOGY, 2002, 36 : 90 - 90
  • [36] Role of hepatitis C virus core antigen assay in hepatitis C care in Africa
    Lemoine, Maud
    Mohamed, Zameer
    Chevaliez, Stephane
    Shimakawa, Yusuke
    Rwegasha, John
    LANCET GASTROENTEROLOGY & HEPATOLOGY, 2018, 3 (04): : 223 - 224
  • [37] Post-transplant recurrent hepatitis C - Immunohistochemical detection of HCV core antigen and possible pathogenic implications.
    Pessoa, MG
    Alves, VA
    Wakamatsu, A
    Maertens, G
    van der Borght, B
    Guanaes, J
    Ferrell, L
    HEPATOLOGY, 2002, 36 (04) : 181A - 181A
  • [38] ANTI-GOR WITHOUT ANTI-HCV CORE IS NOT ASSOCIATED WITH HEPATITIS-C VIREMIA
    BAATH, L
    WIDELL, A
    VOX SANGUINIS, 1994, 66 (02) : 151 - 151
  • [39] Ortho total HCV core antigen assay can aid in the monitoring of liver transplant recipients infected with hepatitis C virus (HCV).
    Regev, A
    Torres, MB
    Hill, MA
    de Medina, M
    Cordero, RA
    Molina, EG
    Reddy, RL
    Tzakis, AG
    Schiff, ER
    HEPATOLOGY, 2002, 36 (04) : 657A - 657A
  • [40] HCV Core-antigen Assay as an Effective Alternative to HCV RNA Quantification in Patients With Hepatitis C
    Vieira, Raquel
    Caldas, Carlos
    Carvalho, Jose Antonio
    Costa, Eliana
    Magalhaes, Tomas Rodrigues
    Queiroga, Felisbina Luisa
    IN VIVO, 2023, 37 (04): : 1498 - 1503