Precision studies using the ABI Prism 3100 Genetic Analyzer for forensic DNA analysis

被引：19
|
作者：
Sgueglia, JB [1 ]
Geiger, S [1 ]
Davis, J [1 ]
机构：
[1] Massachusetts State Police Crime Lab, Sudbury, MA 01776 USA
来源：
ANALYTICAL AND BIOANALYTICAL CHEMISTRY | 2003年 / 376卷 / 08期
关键词：
DNA; STR; capillary electrophoresis; precision;
D O I：
10.1007/s00216-003-1998-7
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Precision studies using the ABI Prism 3100 Genetic Analyzer have been conducted by performing multiple runs of the AmpFlSTR Identifiler allelic ladder, 9947A kit positive control DNA and the 250-base-pair fragment from the internal size standard (GeneScan LIZ-500). Intra-run and inter-run precision data demonstrates the sizing reproducibility of the 3100 instrument for forensic applications. This precision data is utilized to assist in making allele assignments for outlier and variant alleles (i.e., 'off-ladder' alleles). It also provides a means of addressing systematic variations due to temperature fluctuations, polymer (POP-4) or capillary effects, and associated troubleshooting efforts.
引用
收藏
页码：1247 / 1254
页数：8
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