A Single Amino Acid Substitution in the Intervening Region of 129K Protein of Cucumber Green Mottle Mosaic Virus Resulted in Attenuated Symptoms

被引:8
|
作者
Chen, H. [1 ,2 ]
Ino, M. [3 ]
Shimono, M. [3 ]
Wagh, S. G. [1 ]
Kobayashi, K. [1 ]
Yaeno, T. [1 ]
Yamaoka, N. [1 ]
Bai, G. [2 ,4 ]
Nishiguchi, M. [1 ]
机构
[1] Ehime Univ, Fac Agr, Matsuyama, Ehime 7908566, Japan
[2] Kansas State Univ, Dept Agron, Manhattan, KS 66506 USA
[3] Natl Inst Agrobiol Sci, Tsukuba, Ibaraki 3058602, Japan
[4] USDA ARS, Hard Winter Wheat Genet Res Unit, Manhattan, KS 66506 USA
关键词
attenuated virus; biological control; cucumber green mottle mosaic virus; RNA silencing suppression; tobamovirus; COMPLETE NUCLEOTIDE-SEQUENCE; SILENCING SUPPRESSOR; REPLICATION PROTEIN; 126-KDA PROTEIN; RNA-BINDING; GENE; PEPPER; PATHOGENICITY; MOVEMENT; STRAIN;
D O I
10.1094/PHYTO-12-18-0478-FI
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Cucumber green mottle mosaic virus (CGMMV), a member of the genus Tobamovirus, is a major threat to economically important cucurbit crops worldwide. An attenuated strain (SH33b) derived from a severe strain (SH) of CGMMV caused a reduction in the viral RNA accumulation and the attenuation of symptoms, and it has been successfully used to protect muskmelon plants against severe strains in Japan. In this study, we compared GFP-induced silencing suppression by the 129K protein and the methyltransferase domain plus intervening region (MTIR) of the 129K protein between the SH and SH33b strains, respectively. As a result, silencing suppression activity (SSA) in the GFP-silenced plants was inhibited efficiently by the MTIR and 129K protein of SH strain, and it coincided with drastically reduced accumulation of GFP-specific small interfering RNAs (siRNAs) but not by that of SH33b strain. Furthermore, analyses of siRNA binding capability (SBC) by the MTIR of 129K protein and 129K protein using electrophoretic mobility shift assay revealed that SBC was found with the MTIR and 129K protein of SH but not with that of SH33b, suggesting that a single amino acid mutation (E to G) in the MTIR is responsible for impaired SSA and SBC of SH33b. These data suggest that a single amino acid substitution in the intervening region of 129K protein of CGMMV resulted in attenuated symptoms by affecting RNA silencing suppression.
引用
收藏
页码:146 / 152
页数:7
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