Massilia pinisoli sp nov., isolated from forest soil

A Gram-stain-negative, aerobic, motile with a single polar flagellum, non-spore-forming and rod-shaped bacterial strain named T33(T) was isolated from forest soil collected at Kyonggi University, South Korea. The strain was catalase-and oxidase-positive, colonies grew on R2A agar at 32 degrees C. Sequencing of the 16S rRNA gene and phylogenetic analysis revealed that T33(T) represented a member of the genus Massilia and is closely related to Massilia niastensis KACC 12599(T) (98.7% sequence similarity), Massilia aerilata KACC 12505(T) (98.5 %), Massilia tieshanensis KACC 14940(T) (98.4 %), Massilia kyonggiensis KACC 17471(T) (98.1%), Massilia norwichensis LMG 28164(T) (97.7%), Massilia haematophila CCUG 38318(T) (97.4%), Massilia consociata CCUG 58010(T) (97.3%), and Massilia niabensis KACC 12632(T) (97.0%). Ubiquinone Q-8 is the predominant respiratory quinone, and phosphatidylethanolamine, phosphatidylglycerol, and diphosphatidylglycerol are the major polar lipids. The major fatty acids are summed feature 3 (C-16 (:) (1) omega 6c and/or C-16 (:) (1) omega 7c), summed feature 8 (C-18 (:) (1) omega 6c and/or C-18 (:) (1) omega 7c), and C-16 (:) (0). DNA-DNA hybridization revealed <70% relatedness between strain T33(T) and the most closely related type strains. The DNA G+C content of strain T33(T) is 69.4 mol%. Based on physiological and biochemical test results, Massilia pinisoli T33(T) is proposed as a novel species of the genus Massilia. The type strain is T33(T) (=KACC 18748(T) =KEMB 9005-368(T) =JCM 31316(T)).