Evaluation of Two Broadly Used Commercial Methods for Detection of Respiratory Viruses with a Recently Added New Target for Detection of SARS-CoV-2

被引:5
|
作者
Virant, Monika Jevsnik [1 ]
Ursic, Tina [1 ]
Kogoj, Rok [1 ]
Korva, Misa [1 ]
Petrovec, Miroslav [1 ]
Avsic-Zupanc, Tatjana [1 ]
机构
[1] Univ Ljubljana, Fac Med, Inst Microbiol & Immunol, Zaloska 4, Ljubljana 1000, Slovenia
来源
VIRUSES-BASEL | 2022年 / 14卷 / 07期
关键词
SARS-CoV-2; COVID-19; respiratory viruses; validation; molecular assay;
D O I
10.3390/v14071530
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
The clinical symptoms caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) are nonspecific and can be associated with most other respiratory viruses that cause acute respiratory tract infections (ARI). Because the clinical differentiation of COVID-19 patients from those with other respiratory viruses is difficult, the evaluation of automated methods to detect important respiratory viruses together with SARS-CoV-2 seems necessary. Therefore, this study compares two molecular assays for the detection of respiratory viruses, including SARS-CoV-2: the Respiratory Viruses 16-Well Assay (AusDiagnostics, Pty Ltd., Mascot, Australia) and the Allplex (TM) RV Essential Assay coupled with the Allplex (TM)-nCoV Assay (Seegene Inc., Seoul, Korea). The two methods (AusDiagnostics and Alplex (TM)-nCoV Assay SARS-CoV-2) had 98.6% agreement with the reference method, cobas 6800, for the detection of SARS-CoV-2. Agreement between the AusDiagnostics assay and the Alplex (TM) RV Essential Assay for the detection of seven respiratory viruses was 99%. In our experience, the Respiratory Viruses 16-Well Assay proved to be the most valuable and useful medium-throughput method for simultaneous detection of important respiratory viruses and SARS-CoV-2. The main advantages of the method are high specificity for all targets included and their simultaneous detection and medium throughput with the option of having multiple instruments provide a constant run.
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页数:10
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