Electrophoretically deposited nano-structured polyaniline film for glucose sensing

被引：30
|
作者：
Dhand, Chetna [1 ,2 ]
Sumana, G. [1 ]
Datta, Monika [2 ]
Malhotra, B. D. [1 ,3 ]
机构：
[1] Natl Phys Lab, Mat Phys & Engn Div, Biomed Instrumentat Sect, Dept Sci & Technol,Ctr Biomol Elect, New Delhi 110012, India
[2] Univ Delhi, Dept Chem, Delhi 110007, India
[3] Chungnam Natl Univ, Ctr NanoBioEngn & Spintron, Taejon 305764, South Korea
来源：
THIN SOLID FILMS | 2010年 / 519卷 / 03期
关键词：
Electrophoretic deposition; Nano-structured polyaniline; Glucose oxidase; Biosensor; Conducting polymers; CONDUCTING POLYMERS; CARBON NANOTUBES; NANOPARTICLES; NANOFIBERS; BIOSENSORS;
D O I：
10.1016/j.tsf.2010.08.059
中图分类号：
T [工业技术];
学科分类号：
08 ;
摘要：
Electrophoretically deposited nano-structured polyaniline (NS-PANI) film has been utilized for fabrication of glucose biosensor by covalent immobilization of glucose oxidase (GOx) using N-ethyl-N-(3-dimethylaminopropyl) carbodiimide and N-hydroxysuccinimide chemistry. This GOx/NS-PANI/ITO bioelectrode has been characterized using scanning electron microscopy, Fr-IR, UV-Visible spectroscopy and differential pulse voltammetry (DPV) techniques. The response studies carried out on GOx/NS-PANI/ITO bioelectrode using DPV and photometric studies reveal linearity up to 400 mgdL(-1) with sensitivity as 1.05 x 10(-4) mA mg(-1) dL and 3.887 x 10(-5) Abs mg(-1) dL, respectively. The lower value of Michaelis-Menten constant obtained for immobilized GOx (2.1 mM) compared with that of free GOx (5.85 mM) suggests high affinity of enzyme to this matrix. (C) 2010 Elsevier B.V. All rights reserved.
引用
收藏
页码：1145 / 1150
页数：6
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