Involvement of DPY19L3 in Myogenic Differentiation of C2C12 Myoblasts

被引：6

作者：

Mori, Kento ^{[1
]}

Sun, Hongkai ^{[1
]}

Miura, Kazuki ^{[1
]}

Simizu, Siro ^{[1
]}

机构：

[1] Keio Univ, Fac Sci & Technol, Dept Appl Chem, Kohoku Ku, 3-14-1 Hiyoshi, Yokohama, Kanagawa 2238522, Japan

来源：

MOLECULES | 2021年 / 26卷 / 18期

关键词：

C-mannosylation; glycobiology; myotube; myogenic differentiation; MUSCLE-CELL FUSION; C-MANNOSYLATION; ENZYMATIC-ACTIVITY; SECRETION; MANNOSYLTRANSFERASE; IDENTIFICATION; EXPRESSION; R-SPONDIN1; DPY-19;

D O I：

10.3390/molecules26185685

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

DPY19L3 has been identified as a C-mannosyltransferase for thrombospondin type-1 repeat domain-containing proteins. In this study, we focused on the role of DPY19L3 in the myogenic differentiation of C2C12 mouse myoblast cells. We carried out DPY19L3 gene depletion using the CRISPR/Cas9 system. The result showed that these DPY19L3-knockout cells could not be induced for differentiation. Moreover, the phosphorylation levels of MEK/ERK and p70S6K were suppressed in the DPY19L3-knockout cells compared with that of parent cells, suggesting that the protein(s) that is(are) DPY19L3-mediated C-mannosylated and regulate(s) MEK/ERK or p70S6K signaling is(are) required for the differentiation.

引用

页数：9

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