Novel fluorescent probe for the ratiometric detection of β-galactosidase and its application in fruit

被引：18

作者：

Li, Yanan ^{[1
]}

Duan, Ning ^{[1
]}

Wu, Xiaoming ^{[1
]}

Yang, Shaoxiang ^{[1
]}

Tian, Hongyu ^{[1
]}

Sun, Baoguo ^{[1
]}

机构：

[1] Beijing Technol & Business Univ, Beijing Key Lab Flavor Chem, Beijing Adv Innovat Ctr Food Nutr & Human Hlth, Beijing 100048, Peoples R China

来源：

FOOD CHEMISTRY | 2020年 / 328卷

基金：

中国国家自然科学基金;

关键词：

Fluorescent probe; beta-Galactosidase; Fruit; IN-VIVO VISUALIZATION; SENSITIVE DETECTION; GENE-EXPRESSION; REPORTER; HYDROLYSIS; MODULATION; TRACKING; LACTOSE;

D O I：

10.1016/j.foodchem.2020.127112

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

A ratiometric fluorescent probe (Probe 1) was developed for the sensitive detection of beta-galactosidase (beta-gal) activity. Probe 1 detected beta-gal activity in the range 0-1.0 U/mL, with a limit of detection of 0.025 U/mL. In addition, as different activities of beta-gal added, the luminescent intensity of Probe 1 gradually increased, as observed under a 365 nm ultraviolet lamp. Moreover, this method is low-volume, 20 mu L, and time-efficient, 45 min per measurement. Probe 1 was successfully used to measure the beta-gal activity in real fruit samples in a qualitative manner, by the naked eye, fast semi-quantitative manner, by smartphone, or quantitative manner, by fluorescence spectrometer.

引用

页数：7

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