Design of a new hairpin DNAzyme: The activity controlled by TMPyP4

被引：0

作者：

Wu Lei ^{[1
]}

Tong Qian ^{[2
]}

Li Hai-chao ^{[3
]}

Xu Hao-ran ^{[1
]}

Lu Ming ^{[1
]}

Zhang Yue-bin ^{[1
]}

Sun Yu ^{[4
]}

Li Wen-zhao ^{[1
]}

Li Zhengqiang ^{[1
]}

机构：

[1] Jilin Univ, Minist Educ, Key Lab Mol Enzymol & Engn, Changchun 130023, Peoples R China

[2] Jilin Univ, Hosp 1, Dept Cardiol, Changchun 130023, Peoples R China

[3] Jilin Univ, Coll Life Sci, Changchun 130023, Peoples R China

[4] Chang Chun Illness Defend Control Ctr, Changchun, Peoples R China

来源：

AFRICAN JOURNAL OF BIOTECHNOLOGY | 2011年 / 10卷 / 40期

基金：

中国国家自然科学基金;

关键词：

10-23; DNAzyme; hairpin DNAzyme; G-quadruplex structure; TMPyP4; G-QUADRUPLEX; K+ SOLUTION; PORPHYRIN; RNA; TELOMERE; BINDING; LIGAND;

D O I：

暂无

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A new 10-23 hairpin DNAzyme with a G-quadruplex structure stem was designed. The formation and stability of the G-quadruplex structure as the DNAzyme stem in the absence or the presence of TMPyP4 were investigated by UV-visible spectroscopy, circular dichroism (CD) and differential scanning calorimetry (DSC) methods, respectively. The results showed that the stability of this DNAzyme can be enhanced greatly due to the interaction between TMPyP4 and the DNAzyme. The relationship between structural stability and activity of the DNAzyme was studied by in vitro catalytic reaction. The activity of this DNAzyme was regulated by the stability of DNAzyme when TMPyP4 was intercalated into G-quadruplex structure stem. The catalytic activity of the 10-23 hairpin DNAzyme decreased and even inactivated due to the enhanced stability of G-quadruplex structure by TMPyP4 molecules. This DNAzyme is controllable to cleavage substrate and has some potential significance in gene therapy.

引用

页码：7902 / 7910

页数：9

共 50 条

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