Secretion of hen egg white lysozyme from Kluyveromyces lactis

被引：7
|
作者：
Tanaka, R [1 ]
Ishibashi, M [1 ]
Tokunaga, H [1 ]
Tokunaga, M [1 ]
机构：
[1] Kagoshima Univ, Fac Agr, Lab Appl & Mol Microbiol, Kagoshima 8900065, Japan
来源：
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | 2000年 / 64卷 / 12期
关键词：
lysozyme; secretion; Kluyveromyces lactis; shuttle vector;
D O I：
10.1271/bbb.64.2716
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Hen egg white (HEW) lysozyme was correctly processed and efficiently secreted from an alternative yeast, Kluyveromyces lactis. We constructed secretion vectors using PHO5, PGK, and LAC4 promoters, and found that the highest secretion was obtained under the direction of the PGK promoter in non-selective rich medium. K. lactis secreted HEW lysozyme with twofold higher efficiency than S. cerevisiae, estimated by using a K. lactis-S. cerevisiae shuttle vector.
引用
收藏
页码：2716 / 2718
页数：3
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