Characterization of PRLR and PPARGC1A genes in buffalo (Bubalus bubalis)

被引:7
|
作者
Javed, Ruheena [2 ]
Gautam, Sanjeev K. [2 ]
Vijh, Ramesh K.
Tantia, Madhu S. [1 ]
机构
[1] Natl Bur Anim Genet Resources, DNA Sequencing Lab, Karnal 132001, Haryana, India
[2] Kurukshetra Univ, Kurukshetra 132119, Haryana, India
关键词
Bubalus bubalis; buffalo; PRLR gene; PPARGC1A gene; CATTLE; MAP;
D O I
10.1590/S1415-47572011005000037
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
More than 40 million households in India depend at least partially on livestock production. Buffaloes are one of the major milk producers in India. The prolactin receptor (PRLR) gene and peroxisome proliferators activated receptor-gamma coactivator 1-alpha (PPARGC1A) gene are reportedly associated with milk protein and milk fat yields in Bos taurus. In this study, we sequenced the PRLR and PPARGC1A genes in the water buffalo Bubalus bubalis. The PRLR and PPARGC1A genes coded for 581 and 819 amino acids, respectively. The B. bubalis PRLR gene differed from the corresponding Bos taurus at 21 positions and four differences with an additional arginine at position 620 in the PPARGC1A gene were found in the amino acid sequence. All of the changes were confirmed by cDNA sequencing. Twelve buffalo-specific single nucleotide polymorphisms (SNPs) were identified in both genes, with five of them being non-synonymous.
引用
收藏
页码:592 / 594
页数:3
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