Ultrastructural Evaluation of the Human Oocyte at the Germinal Vesicle Stage during the Application of Assisted Reproductive Technologies

被引:6
|
作者
Palmerini, Maria Grazia [1 ]
Antonouli, Sevastiani [1 ]
Macchiarelli, Guido [1 ]
Cecconi, Sandra [1 ]
Bianchi, Serena [1 ]
Khalili, Mohammad Ali [2 ]
Nottola, Stefania Annarita [3 ]
机构
[1] Univ Aquila, Dept Life Hlth & Environm Sci, I-67100 Laquila, Italy
[2] Shahid Sadoughi Univ Med Sci, Yazd Inst Reprod Sci, Dept Reprod Biol, Yazd 97514, Iran
[3] Univ Roma La Sapienza, Dept Anat Histol Forens Med & Orthoped Sci, I-00161 Rome, Italy
关键词
oocyte; germinal vesicle; human; electron microscopy; assisted reproductive technologies (ARTs); IN-VITRO MATURATION; HUMAN IMMATURE OOCYTES; NATURAL CYCLE IVF; DEVELOPMENTAL COMPETENCE; OVARIAN STIMULATION; MITOCHONDRIAL DISTRIBUTION; CLINICAL-APPLICATION; MATURE OOCYTES; CRYOPRESERVATION; FERTILIZATION;
D O I
10.3390/cells11101636
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
After its discovery in 1825 by the physiologist J.E. Purkinje, the human germinal vesicle (GV) attracted the interest of scientists. Discarded after laparotomy or laparoscopic ovum pick up from the pool of retrieved mature oocytes, the leftover GV was mainly used for research purposes. After the discovery of Assisted Reproductive Technologies (ARTs) such as in vitro maturation (IVM), in vitro fertilization and embryo transfer (IVF-ET) and intracytoplasmic sperm injection (ICSI), its developing potential was explored, and recognized as an important source of germ cells, especially in the case of scarce availability of mature oocytes for pathological/clinical conditions or in the case of previous recurrent implantation failure. We here review the ultrastructural data available on GV-stage human oocytes and their application to ARTs.
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页数:16
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