Alpha-momorcharin inhibits HIV-1 replication in acutely but not chronically infected T-lymphocytes

被引：0

作者：

Zheng, YT

Ben, KL ^{[1
]}

Jin, SW

机构：

[1] Chinese Acad Sci, Kunming Inst Zool, Kunming 650223, Peoples R China

[2] Chinese Acad Sci, Shanghai Inst Organ Chem, Shanghai 200032, Peoples R China

来源：

ACTA PHARMACOLOGICA SINICA | 1999年 / 20卷 / 03期

关键词：

alpha-momorcharin; ribosome-inactivating proteins; HIV; antigen p24; giant cells; T-lymphocytes; anti-HIV agents; cytotoxicity;

D O I：

暂无

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

AIM: To identify the anti-human immunodeficiency virus type 1 (HIV-1) activities of alpha-momorcharin ( alpha-MMC) from Momordica charantia in acutely and chronically infected lymphocytes. METHODS: The anti-HIV activities of alpha-MMC were examined by 1) the inhibition of syncytia formation induced by HIV-1 III B; 2) reduction of p24 core antigen expression level and decrease in numbers of HIV antigen positive cells in acutely and chronically infected cultures. The cytotoxic effects of alpha-MMC was tested by trypan blue dye exclusion or colorimetric MTT assay. RESULTS: alpha-MMC was found to obviously inhibit HIV-1 III B-inducing C8166 syncytia formation and markedly reduced both expression of p24 core antigen and the numbers of HIV antigen positive cells in acutely but not chronically HTV-1-infected culture. The median effective concentration (EC50) in these assays were 0.016, 0.07, and 0.32 mg.L-1, respectively. CONCLUSION: alpha-MMC is a unique component of momorcharin with anti-HIV activity, and markedly inhibited HIV-1 replication in acutely but not chronically HIV-1-infected T-lymphocytes.

引用

页码：239 / 243

页数：5

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