Sulfated modification of epimedium polysaccharide and effects of the modifiers on cellular infectivity of IBDV

被引：120
|
作者：
Lu, Yu [1 ]
Wang, Deyun [1 ]
Hu, Yuanliang [1 ]
Huang, Xiaoyan [1 ]
Wang, Junmin [1 ]
机构：
[1] Nanjing Agr Univ, Coll Vet Med, Inst Tradit Chinese Vet Med, Nanjing 210095, Peoples R China
来源：
CARBOHYDRATE POLYMERS | 2008年 / 71卷 / 02期
基金：
中国国家自然科学基金;
关键词：
EPS; sulfated modification; IBDV; cellular infectivity;
D O I：
10.1016/j.carbpol.2007.05.024
中图分类号：
O69 [应用化学];
学科分类号：
081704 ;
摘要：
Nine modification conditions were designed to sulfate epimedium polysaccharide (EPS) by chlorosulfonic acid-pyridine method according to orthogonal test. and nine sulfated EPS (sEPS), sEPS(1), sEPS(2), sEPS(3), sEPS(4), sEPS(5), sEPS(6), sEPS(7), sEPS(8) and sEPS(9), were obtained and their effects on IBDV infecting chick embryo fibroblast (CEF) was compared by MTT assay. The results showed that modified sEPS had significantly enhanced CEF resisting IBDV infection in comparison of non-sulfated EPS, especially, sEPS(2) and sEPS(5) presented better effects. which there were certain relationships in the degree Of Sulfate group (DS) and carbohydrate content. The optimum modification conditions were the reaction temperature of 80 degrees C, the ratio of chlorosulfonic acid to pyridine of 1:8 and the reaction time of 2 h. (C) 2007 Elsevier Ltd. All rights reserved.
引用
收藏
页码：180 / 186
页数：7
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